Description and characterization of Borrelia genospecies in infected patients in the Western region of Venezuela

IF 2.5 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2025-06-27 DOI:10.1016/j.actatropica.2025.107707

José Goyo Rivas , Mariolly Rendon , Solsiree Maldonado , Lousdy Carolina Gómez Abraham , Darling Stella Morales Andará , Delsy Dávila-Vera , Rosa Virginia Mendoza-Briceño , Siham Salmen

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引用次数: 0

Abstract

Background

Lyme disease, or Lyme borreliosis (LB), is the most prevalent vector-borne disease, representing over 75 % of tick-borne illnesses, with approximately 700,000 cases reported annually in the United States and Europe. No studies in Venezuela have explored the presence or genospecies diversity of Borrelia spp. This study aimed to detect and characterize Borrelia spp. circulating in Venezuela.

Methods

A cohort of 1140 patients was analyzed using microscopy, real-time PCR, enzyme immunoassay (EIA), and Western blot to identify Borrelia spp. and associated hemoparasites.

Results

Borrelia spp. were detected in 188 (16.49 %) patients, with 130 (69.14 %) exhibiting coinfections with other hemoparasites, such as Babesia spp. and Ehrlichia spp. The predominant genospecies was B. garinii (43.5 % IgM+, 13 % IgG+), followed by B. spielmanii (21.7 % IgM+, 4.3 % IgG+), B. afzelii (19.6 % IgM+, 2.2 % IgG+), B. burgdorferi sensu stricto (Bbss) (10.9 % IgM+, 6.5 % IgG+), and B. bavariensis (2.2 % IgM+, 4.3 % IgG+). Co-occurrence of multiple genospecies was observed in 36.73 % of cases.

Conclusions

This study in Venezuela confirms the presence of Lyme borreliosis and highlights the diversity of circulating genospecies. The use of sensitive, broad-spectrum diagnostic tools is critical to reducing false negatives and ensuring timely diagnosis, thereby improving patient outcomes.

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委内瑞拉西部地区感染患者伯氏疏螺旋体基因种的描述和特征

背景：莱姆病或莱姆博氏螺旋体病（LB）是最普遍的媒介传播疾病，占蜱传疾病的75%以上，在美国和欧洲每年报告约70万例。委内瑞拉目前尚未有研究发现博氏疏螺旋体的存在及其基因多样性，本研究旨在对委内瑞拉流行的博氏疏螺旋体进行检测和鉴定。方法：采用显微镜、实时荧光定量PCR、酶免疫分析（EIA）和Western blot对1140例患者进行分析，鉴定伯氏疏螺旋体及其相关血液寄生虫。结果188例（16.49%）患者检出伯氏疏螺旋体，其中130例（69.14%）与巴贝斯虫、埃利希氏体等其他血液寄生虫共感染，优势种为加里尼布氏螺旋体（43.5% IgM+, 13% IgG+），其次为spielmanb （21.7% IgM+, 4.3% IgG+）、阿夫zelii b （19.6% IgM+, 2.2% IgG+）、burgdorferi （10.9% IgM+, 6.5% IgG+）和巴伐利亚布氏螺旋体（2.2% IgM+, 4.3% IgG+）。多基因种共出现的病例占36.73%。结论：委内瑞拉的这项研究证实了莱姆病的存在，并强调了流行基因种的多样性。使用敏感的广谱诊断工具对于减少假阴性和确保及时诊断，从而改善患者预后至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.