Brazilin Inhibits the Proliferation of Non-Small Cell Lung Cancer by Regulating the STING/TBK1/IRF3 Pathway

Abstract

Non-small cell lung cancer (NSCLC) stands as a predominant cause of cancer-related mortality worldwide. Brazilin, an active isoflavonoid compound derived from Chinese herbs, has displayed anti-cancer properties across various cancer cell lines. However, the precise anti-tumour mechanism of Brazilin in NSCLC remains incompletely understood. In this paper, we demonstrated that Brazilin treatment significantly reduced the proliferation of NSCLC cells and induced apoptosis. Additionally, Brazilin caused G2 cell cycle arrest in NSCLC cells, characterised by decreased expression of Cyclin B1 and increased expression of P21. Brazilin also induced mitochondrial dysfunction and ROS production in NSCLC cells. Mechanistically, Brazilin treatment significantly activated the STING pathway and upregulated the expression of CXCL10, CXCL9, and CCL5 in NSCLC cell lines. Notably, the inhibition of the STING pathway with H-151 enhances cell viability, suggesting STING is involved in Brazilin-induced apoptosis. These findings underscore Brazilin as a promising anti-cancer agent for NSCLC.