Sildenafil effect on testosterone-induced prostate hypertrophy and relaxation of urinary bladder neck muscles

Q1 Environmental Science
Qasim A. El-Dwairi , Karem H. Alzoubi , Rania Mahafdeh
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Abstract

Background

Previous studies have demonstrated the expression of phosphodiesterase-5 receptors in prostate tissue. In this study, we investigated the efficacy of sildenafil citrate in testosterone-induced benign prostate hyperplasia (BPH) in rabbits.

Methods

Prostate hyperplasia was induced using testosterone propionate for 8 weeks. Rabbits were divided into two groups: control and experimental. The experimental group was further subdivided into two subgroups: one subgroup was sacrificed after testosterone induction, while the other subgroup received sildenafil (5 mg/kg/day) via intragastric intubation for 8 weeks. The weight of the prostate and relaxation of the bladder neck muscle were assessed. Organ bath experiments evaluated the effect of sildenafil on phenylephrine-precontracted bladder neck muscle strips.

Results

The mean prostate weight was reduced by 65.34 % after 8 weeks of treatment with sildenafil in animals with BPH. Sildenafil induced significant smooth muscle relaxation of the phenylephrine-contracted bladder neck muscle strips. The mean relaxation value was 2.11 ± 0.13, representing a 32.8 % reduction in contraction percentage. Maximal relaxation was produced at 5.0 × 10⁻⁶ M of sildenafil. Sildenafil induced significant relaxation of the phenylephrine-contracted bladder neck muscle strips. Adding the nitric oxide synthase inhibitor L-NAME inhibited relaxation, whereas sodium nitroprusside, a nitric oxide donor, increased it. Histopathological analysis showed increased papillary projections, acinar areas, and epithelial thickness in the testosterone-treated group. Sildenafil treatment reversed the hypertrophic and hyperplastic changes.

Conclusions

The results indicate that sildenafil may provide a dual function in the treatment of erectile dysfunction and the relief of urinary tract complications associated with prostatic hypertrophy.
西地那非对睾酮所致前列腺肥大及膀胱颈部肌肉松弛的影响
前期研究证实磷酸二酯酶-5受体在前列腺组织中表达。在这项研究中,我们研究了柠檬酸西地那非对睾丸激素诱导的兔良性前列腺增生(BPH)的疗效。方法采用丙酸睾酮诱导前列腺增生8周。将家兔分为对照组和实验组。实验组进一步分为2个亚组,一个亚组在睾酮诱导后处死,另一个亚组给予西地那非(5 mg/kg/天)灌胃,持续8周。评估前列腺重量和膀胱颈肌松弛程度。器官浴实验评价西地那非对苯肾上腺素-预收缩膀胱颈肌条的影响。结果经西地那非治疗8周后,前列腺增生动物平均前列腺重量降低65.34% %。西地那非引起苯肾上腺素收缩的膀胱颈肌条明显的平滑肌松弛。平均松弛值为2.11 ± 0.13,收缩百分比减少32.8% %。最大松弛是5.0 × 10⁻26 M的西地那非。西地那非诱导苯肾上腺素收缩的膀胱颈肌条明显松弛。添加一氧化氮合酶抑制剂L-NAME抑制松弛,而硝普钠(一氧化氮供体)则增加松弛。组织病理学分析显示,睾酮治疗组的乳头状突起、腺泡面积和上皮厚度增加。西地那非治疗逆转了肥厚和增生性改变。结论西地那非具有治疗勃起功能障碍和减轻前列腺肥大相关尿路并发症的双重作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Toxicology Reports
Toxicology Reports Environmental Science-Health, Toxicology and Mutagenesis
CiteScore
7.60
自引率
0.00%
发文量
228
审稿时长
11 weeks
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