Effects of acute treadmill running following administration of lipopolysaccharide on subsequent changes in microglial activation and depressive-like behavior in rats

IF 2.6 3区心理学 Q2 BEHAVIORAL SCIENCES

Behavioural Brain Research Pub Date : 2025-06-30 DOI:10.1016/j.bbr.2025.115718

Shuntaro Sugimoto, Misaki Yokoshi, Takumi Maruyama, Seiichiro Amemiya, Ichiro Kita

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引用次数: 0

Abstract

The mechanisms underlying the beneficial effects of physical exercise on the brain, including its antidepressant properties, have yet to be fully elucidated. Recently, there has been growing interest that the beneficial effects of physical exercise may be related to its ability to reduce neuroinflammation by modulating microglial activation in the brain. However, no studies have investigated the relationship between microglial activation and depressive symptoms in the context of exercise intervention. The goal of the study was to assess if exercise would reduce depression-like behaviors and microglia activation induced by lipopolysaccharide (LPS). Here, we examined whether a bout of treadmill running (15 m/min, 30 min) performed 2 h after the administration of LPS (0.83 mg/mL/kg, i.p.) would affect subsequent changes in microglial activation and depressive-like behavior in rats. Microglial activation was assessed by alterations in microglial morphology (somatic cell size) in the dorsal raphe nucleus (DRN), hippocampal dentate gyrus (DG) and hypothalamic paraventricular nucleus (PVN), which were selected based on their involvement in stress regulation and the pathophysiology of depression, performing immunohistochemistry for ionized calcium-binding adapter molecule 1 (Iba1). In addition, we performed a forced swim test (FST) in the LPS-treated rats to assess the antidepressant effect of physical exercise on inflammation-induced depression. The increase of the somatic area of Iba1-positive cells in the DRN and DG at 24 h after LPS administration was significantly attenuated by a bout of acute and mild treadmill running performed 2 h after LPS administration. In addition, the LPS-induced increase of immobility time in the FST was significantly suppressed by the acute treadmill running. These results suggest that mild physical exercise can suppress microglia-induced neuroinflammation and exert an antidepressant effect against inflammation-induced depression.

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脂多糖对大鼠小胶质细胞激活和抑郁样行为的影响

体育锻炼对大脑有益作用的潜在机制，包括其抗抑郁特性，尚未得到充分阐明。最近，人们越来越感兴趣的是，体育锻炼的有益作用可能与它通过调节大脑中的小胶质细胞激活来减少神经炎症的能力有关。然而，在运动干预的背景下，没有研究调查小胶质细胞激活与抑郁症状之间的关系。这项研究的目的是评估运动是否会减少由脂多糖（LPS）诱导的抑郁样行为和小胶质细胞激活。在这里，我们研究了在给药LPS(0.83 mg/mL/kg, i.p.) 2 小时后进行一次跑步（15 m/min, 30 min）是否会影响大鼠小胶质细胞激活和抑郁样行为的后续变化。通过中缝背核（DRN）、海马齿状回（DG）和下丘脑室旁核（PVN）的小胶质细胞形态（体细胞大小）的改变来评估小胶质细胞的激活，这些小胶质细胞是根据它们参与应激调节和抑郁症的病理生理而选择的，对离子钙结合适配分子1 （Iba1）进行免疫组化。此外，我们对lps治疗的大鼠进行了强迫游泳试验（FST），以评估体育锻炼对炎症性抑郁症的抗抑郁作用。LPS给药后2 h进行急性和轻度跑步，可显著减弱LPS给药后24 h DRN和DG中iba1阳性细胞的体面积增加。此外，lps诱导的FST静止时间的增加被急性跑步机运动显著抑制。提示轻度体育锻炼可抑制小胶质细胞诱导的神经炎症，对炎症性抑郁具有抗抑郁作用。

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来源期刊

Behavioural Brain Research 医学-行为科学

CiteScore

5.60

自引率

0.00%

发文量

383

审稿时长

61 days

期刊介绍： Behavioural Brain Research is an international, interdisciplinary journal dedicated to the publication of articles in the field of behavioural neuroscience, broadly defined. Contributions from the entire range of disciplines that comprise the neurosciences, behavioural sciences or cognitive sciences are appropriate, as long as the goal is to delineate the neural mechanisms underlying behaviour. Thus, studies may range from neurophysiological, neuroanatomical, neurochemical or neuropharmacological analysis of brain-behaviour relations, including the use of molecular genetic or behavioural genetic approaches, to studies that involve the use of brain imaging techniques, to neuroethological studies. Reports of original research, of major methodological advances, or of novel conceptual approaches are all encouraged. The journal will also consider critical reviews on selected topics.