Galectin-1 from blunt snout bream (Megalobrama amblycephala) participates in anti-bacterial immune response through mediating pathogen recognition

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-06-29 DOI:10.1016/j.fsi.2025.110536

Zuzhen Wang , Zongqiang Chen , Jing Wang , Bing Liu , Hongyang Sun , Jinpeng Yan

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Abstract

Galectins are involved in immune response against pathogenic microorganisms. Here, a prototypic galectin-1 (MaGal-1) from blunt snout bream (Megalobrama amblycephala) was identified and characterized. MaGal-1 comprises an open reading frame spanning 405 bp and encodes a deduced protein of 134 amino acids, containing one single carbohydrate recognition domain, in which two strictly conserved motifs were detected. MaGal-1 transcript was ubiquitously detected in detected tissues with higher expression in muscle, liver and spleen, and its expression was up-regulated in head-kidney liver and spleen after injection with LPS and Aeromonas hydrophila. Sublocalization analysis revealed that MaGal-1 was mainly distributed in the cytoplasm and nucleus of EPC cells. The rMaGal-1 agglutinated markedly Staphylococcus aureus, Escherichia coli and A. hydrophila. Notably, rMaGal-1 bound five different bacteria, which was inhibited by LPS, glucose and trehalose. Besides that, rMaGal-1 could remarkably inhibit the growth of three types of bacteria in vitro. Furthermore, rMaGal-1 destroyed bacterial cell membrane as confirmed by PI staining and SEM observation. Collectively, these findings indicated that MaGal-1 recognized and killed a wide range of microbes and played the vital role in innate immune, which sets the stage for further studies of antibacterial functions of galectins in teleost fish.

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钝口鲷（Megalobrama amblycephala）半乳糖凝集素-1通过介导病原体识别参与抗细菌免疫应答

半凝集素参与了对病原微生物的免疫反应。本文从钝口鲷（Megalobrama amblycephala）中鉴定并表征了一个原型半乳糖凝集素-1 （MaGal-1）。MaGal-1包含一个全长405 bp的开放阅读框，编码一个包含134个氨基酸的推断蛋白，包含一个单一的碳水化合物识别结构域，其中检测到两个严格保守的基序。MaGal-1转录本在检测组织中普遍存在，在肌肉、肝脏和脾脏中表达较高，注射LPS和嗜水气单胞菌后，其在头肾肝和脾脏中的表达上调。亚定位分析显示，MaGal-1主要分布在EPC细胞的细胞质和细胞核中。rMaGal-1对金黄色葡萄球菌、大肠杆菌和嗜水芽胞杆菌有明显的凝集作用。值得注意的是，rMaGal-1结合了5种不同的细菌，被LPS、葡萄糖和海藻糖抑制。此外，rMaGal-1在体外对三种细菌的生长均有显著抑制作用。此外，通过PI染色和扫描电镜观察证实，rMaGal-1对细菌细胞膜有破坏作用。总之，这些发现表明MaGal-1能够识别和杀死多种微生物，并在先天免疫中发挥重要作用，这为进一步研究凝集素在硬骨鱼中的抗菌功能奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.