Weilong Tu , Meng Sun , Tianyi Lu , Yuxian Chen , Yuxuan Zhou , Cong Zhang , Zhonghua Ni , Xiao Li , Tao Hu
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引用次数: 0
Abstract
Chronic stress exerts detrimental effects on both physical and mental wellbeing, thereby necessitating the quantitative and real-time monitoring of stress biomarkers, such as cortisol. To address this, a non-invasive, wearable electrochemical aptasensor based on MXene and gold nanoparticles (Mxene/AuNPs) modified screen-printed electrodes (SPEs) is proposed to detect cortisol in human sweat. Conformationally altered aptamers, with methylene blue (MB) as an electrochemical indicator, are linked to AuNPs via gold-sulfur bonds to capture cortisol. Mxene/AuNPs not only increase the specific surface area of the working electrode but also facilitate the immobilization of the aptamer. Moreover, an integrated microfluidic sampler is integrated with this aptasensor to ensure efficient sweat collection, which could effectively prevent sweat evaporation and contamination. Finally, the performance of this aptasensor is evaluated through the differential pulse voltammetry (DPV) technique, exhibiting high specificity ranging from 0.5 to 500 ng/ml (1.38–1379 nM) with a low detection limit of 0.1 ng/ml. This work demonstrates a practical strategy for developing cost-effective and scalable wearable sensors for sweat-based biomarker detection.
期刊介绍:
Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.