Design and application of HBT based probes: achieving color changing function and drug release through cascade reaction and photoactivation

IF 4.2 3区工程技术 Q2 CHEMISTRY, APPLIED

Dyes and Pigments Pub Date : 2025-05-31 DOI:10.1016/j.dyepig.2025.112923

Wenyun Pan , Yuyu Yang , Qiao Liang , Fei Zhang , Qianshan Shao , Chunxiao Li , Zhengxi Hu , Guang Chen , Baolei Fan

{"title":"Design and application of HBT based probes: achieving color changing function and drug release through cascade reaction and photoactivation","authors":"Wenyun Pan , Yuyu Yang , Qiao Liang , Fei Zhang , Qianshan Shao , Chunxiao Li , Zhengxi Hu , Guang Chen , Baolei Fan","doi":"10.1016/j.dyepig.2025.112923","DOIUrl":null,"url":null,"abstract":"<div><div>Cell fluorescence visualization is a promising method for disease detection, which can realize dynamic monitoring of living cells and provide the possibility for early diagnosis and treatment of diseases. However, most probes have low targeting and lack precision. Dual recognition strategy uses two biomarkers as trigger factors, which can improve the specificity and accuracy of diagnosis and treatment. In this paper, cell fluorescence technology is combined with this strategy, and a photosensitive drug-release probe (MUS-AOR), also referred to as “Light-Sensitive Probe”, is reported. It is activated by the cascade reaction of hydrogen peroxide tyrosinase (H<sub>2</sub>O<sub>2</sub>-Tyr.) and excited by light energy in a specific range (<em>hν</em> ≥ 410 nm), which can separate the probe molecule from the carried drug and achieve the targeted drug delivery process. In this process, the fluorescence changes blue-green and returns to blue after photoexcitation to release the drug. The color-changing function allows the recognition process to be more visible, enabling real-time monitoring of drug release information, preventing excessive hydrogen peroxide, detecting tyrosinase abnormalities, and optimizing the storage and release of anticancer drugs. Notably, the probe has a low detection limit (0.0041 U/mL) and also demonstrated strong phototoxicity in breast tumor cells, and we hope that this breakthrough can improve early detection and personalized treatment of breast cancer.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"242 ","pages":"Article 112923"},"PeriodicalIF":4.2000,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dyes and Pigments","FirstCategoryId":"88","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0143720825002931","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

Cell fluorescence visualization is a promising method for disease detection, which can realize dynamic monitoring of living cells and provide the possibility for early diagnosis and treatment of diseases. However, most probes have low targeting and lack precision. Dual recognition strategy uses two biomarkers as trigger factors, which can improve the specificity and accuracy of diagnosis and treatment. In this paper, cell fluorescence technology is combined with this strategy, and a photosensitive drug-release probe (MUS-AOR), also referred to as “Light-Sensitive Probe”, is reported. It is activated by the cascade reaction of hydrogen peroxide tyrosinase (H₂O₂-Tyr.) and excited by light energy in a specific range (hν ≥ 410 nm), which can separate the probe molecule from the carried drug and achieve the targeted drug delivery process. In this process, the fluorescence changes blue-green and returns to blue after photoexcitation to release the drug. The color-changing function allows the recognition process to be more visible, enabling real-time monitoring of drug release information, preventing excessive hydrogen peroxide, detecting tyrosinase abnormalities, and optimizing the storage and release of anticancer drugs. Notably, the probe has a low detection limit (0.0041 U/mL) and also demonstrated strong phototoxicity in breast tumor cells, and we hope that this breakthrough can improve early detection and personalized treatment of breast cancer.

查看原文本刊更多论文

基于HBT的探针设计与应用：通过级联反应和光活化实现变色功能和药物释放

细胞荧光可视化是一种很有前途的疾病检测方法，可以实现对活细胞的动态监测，为疾病的早期诊断和治疗提供可能。然而，大多数探针具有低瞄准和缺乏精度。双识别策略使用两种生物标志物作为触发因子，可以提高诊断和治疗的特异性和准确性。本文将细胞荧光技术与该策略相结合，报道了一种光敏药物释放探针（MUS-AOR），也称为“光敏探针”。由过氧化氢酪氨酸酶（H2O2-Tyr）级联反应激活，在特定范围内（hν≥410 nm）受光能激发，可将探针分子与携带药物分离，实现靶向给药过程。在此过程中，荧光变为蓝绿色，光激发后又恢复为蓝色，释放药物。变色功能使识别过程更加清晰可见，实时监测药物释放信息，防止过氧化氢过量，检测酪氨酸酶异常，优化抗癌药物的储存和释放。值得注意的是，该探针的检出限较低（0.0041 U/mL），并且在乳腺肿瘤细胞中表现出较强的光毒性，我们希望这一突破能够促进乳腺癌的早期发现和个性化治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.