{"title":"Investigation on thyroglobulin and myoglobin using graphene-enhanced Raman spectroscopy as a tool†","authors":"Anamika Sharma and Venkata Ramanaiah Dantham","doi":"10.1039/D5NR00800J","DOIUrl":null,"url":null,"abstract":"<p >Herein, for the first time, we report the experimental investigation on thyroglobulin (Tg) and myoglobin (Mb) using graphene-enhanced Raman spectroscopy as a tool. Tg is the protein precursor of thyroid hormones, while Mb can be used as an early cardiac protein biomarker. In the beginning, the absorption properties of Tg and Mb are investigated with the help of a UV-visible spectrophotometer. Later, Tg and Mb are dispersed over two different glass substrates by drop-casting 10 μL of protein solutions with a concentration of 1 μM and their conventional Raman scattering (CRS) spectra are recorded. However, none of the vibrational modes are significant in their CRS spectra. Therefore, the graphene-enhanced Raman scattering (GERS) spectra of Tg and Mb are recorded with a high signal-to-noise ratio. The number and intensity of the vibrational modes in the GERS spectra are found to strongly depend upon the probed location. Finally, the assignment of all the observed vibrational modes is performed.</p>","PeriodicalId":92,"journal":{"name":"Nanoscale","volume":" 29","pages":" 17256-17264"},"PeriodicalIF":5.1000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/nr/d5nr00800j?page=search","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanoscale","FirstCategoryId":"88","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/nr/d5nr00800j","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Herein, for the first time, we report the experimental investigation on thyroglobulin (Tg) and myoglobin (Mb) using graphene-enhanced Raman spectroscopy as a tool. Tg is the protein precursor of thyroid hormones, while Mb can be used as an early cardiac protein biomarker. In the beginning, the absorption properties of Tg and Mb are investigated with the help of a UV-visible spectrophotometer. Later, Tg and Mb are dispersed over two different glass substrates by drop-casting 10 μL of protein solutions with a concentration of 1 μM and their conventional Raman scattering (CRS) spectra are recorded. However, none of the vibrational modes are significant in their CRS spectra. Therefore, the graphene-enhanced Raman scattering (GERS) spectra of Tg and Mb are recorded with a high signal-to-noise ratio. The number and intensity of the vibrational modes in the GERS spectra are found to strongly depend upon the probed location. Finally, the assignment of all the observed vibrational modes is performed.
期刊介绍：
Nanoscale is a high-impact international journal, publishing high-quality research across nanoscience and nanotechnology. Nanoscale publishes a full mix of research articles on experimental and theoretical work, including reviews, communications, and full papers.Highly interdisciplinary, this journal appeals to scientists, researchers and professionals interested in nanoscience and nanotechnology, quantum materials and quantum technology, including the areas of physics, chemistry, biology, medicine, materials, energy/environment, information technology, detection science, healthcare and drug discovery, and electronics.
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