Fluorescent Polyamines for Efficient DNA Condensation and Cellular Uptake.

IF 12.1 2区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Small Pub Date : 2025-07-01 DOI:10.1002/smll.202503144

Yogesh Dubey,Haritha Dilip,Sivapriya Kirubakaran,Sriram Kanvah

引用次数: 0

Abstract

Polyamine-based carriers, including spermine and spermidine, offer significant potential due to their ability to bind DNA and facilitate cellular uptake. In this study, four fluorophore-tagged spermidine and spermine derivatives (S1C, S2C, S1D, S2D) linked by a non-cleavable linker are synthesized and evaluated for their ability to condense DNA and mediate gene delivery. Spectroscopic and bio-AFM studies confirm the DNA condensation, with S2C exhibiting selective lysosomal targeting and photostability for up to 2 h. Further validation using an RFP-tagged plasmid confirms S2C's effective DNA internalization. This approach offers a promising, straightforward alternative for gene delivery, addressing key limitations of conventional methods. However, further optimization is needed to improve gene release and enhance the therapeutic efficacy.

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高效DNA凝聚和细胞摄取的荧光多胺。

基于多胺的载体，包括精胺和亚精胺，由于其结合DNA和促进细胞摄取的能力，提供了巨大的潜力。在这项研究中，合成了四种荧光团标记的精胺和精胺衍生物（S1C, S2C, S1D, S2D），它们通过一个不可切割的连接体连接，并评估了它们浓缩DNA和介导基因传递的能力。光谱和生物afm研究证实了DNA凝聚，S2C表现出选择性溶酶体靶向和长达2小时的光稳定性。使用rfp标记的质粒进一步验证证实了S2C有效的DNA内化。这种方法为基因传递提供了一种有希望的、直接的替代方法，解决了传统方法的关键局限性。然而，需要进一步优化以改善基因释放，提高治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Small 工程技术-材料科学：综合

CiteScore

17.70

自引率

3.80%

发文量

1830

审稿时长

2.1 months

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