Seasonal Host Shifts for Legionella Within an Industrial Water-Cooling System

IF 3.6 4区 生物学 Q2 ENVIRONMENTAL SCIENCES
Suzanne Crull, Emlyn Hammer, Allison E. Mann, Lauren M. O'Connell, Ashlyn Soule, Elizabeth Griffith, Thomas Blouin, Robin L. Brigmon, Vincent P. Richards
{"title":"Seasonal Host Shifts for Legionella Within an Industrial Water-Cooling System","authors":"Suzanne Crull,&nbsp;Emlyn Hammer,&nbsp;Allison E. Mann,&nbsp;Lauren M. O'Connell,&nbsp;Ashlyn Soule,&nbsp;Elizabeth Griffith,&nbsp;Thomas Blouin,&nbsp;Robin L. Brigmon,&nbsp;Vincent P. Richards","doi":"10.1111/1758-2229.70132","DOIUrl":null,"url":null,"abstract":"<p><i>Legionella</i> is a genus of environmental bacteria containing pathogenic species such as <i>Legionella pneumophila</i> that are responsible for Legionnaires' disease, a potentially fatal respiratory infection. Disease aetiology can involve <i>Legionella</i> replication intracellularly within protists and this study aimed to characterise the <i>Legionella</i>-protist relationship to develop novel outbreak prevention targets. Water and sediment samples were collected from a water-cooling tower in South Carolina over a 6-month period. Concomitantly, multiple environmental parameters were recorded. Bacterial and eukaryotic communities were characterised using 16S rRNA gene V4 region and a 252 bp fragment of 18S rRNA gene, respectively. Co-occurrence network analyses were performed to elucidate <i>Legionella</i>-protist correlations through time. We found that <i>Legionella</i> correlated with different protists as the seasons progressed. <i>Acanthamoeba</i> correlated with <i>Legionella</i> in early spring followed by <i>Vannella</i> and <i>Korotnevella</i> in late spring and early summer, and were joined by <i>Echinamoeba</i> in mid-summer. <i>Vannella</i> and <i>Acanthamoeba</i> are known potential hosts for <i>Legionella</i>, while <i>Korotnevella</i> is a potential undocumented host. Of the environmental parameters, temperature showed strong correlation with protists genera, suggesting that <i>Legionella</i> abundance was driven by temperature-dependent protist availability. Our results highlight ecological shifts that are associated with elevated <i>Legionella</i> levels, which offers potential targets to help predict and prevent disease outbreaks.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"17 4","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1758-2229.70132","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Microbiology Reports","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1758-2229.70132","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Legionella is a genus of environmental bacteria containing pathogenic species such as Legionella pneumophila that are responsible for Legionnaires' disease, a potentially fatal respiratory infection. Disease aetiology can involve Legionella replication intracellularly within protists and this study aimed to characterise the Legionella-protist relationship to develop novel outbreak prevention targets. Water and sediment samples were collected from a water-cooling tower in South Carolina over a 6-month period. Concomitantly, multiple environmental parameters were recorded. Bacterial and eukaryotic communities were characterised using 16S rRNA gene V4 region and a 252 bp fragment of 18S rRNA gene, respectively. Co-occurrence network analyses were performed to elucidate Legionella-protist correlations through time. We found that Legionella correlated with different protists as the seasons progressed. Acanthamoeba correlated with Legionella in early spring followed by Vannella and Korotnevella in late spring and early summer, and were joined by Echinamoeba in mid-summer. Vannella and Acanthamoeba are known potential hosts for Legionella, while Korotnevella is a potential undocumented host. Of the environmental parameters, temperature showed strong correlation with protists genera, suggesting that Legionella abundance was driven by temperature-dependent protist availability. Our results highlight ecological shifts that are associated with elevated Legionella levels, which offers potential targets to help predict and prevent disease outbreaks.

Abstract Image

工业水冷系统中军团菌的季节性宿主变化
军团菌是一种环境细菌属,含有致病性物种,如嗜肺军团菌,可导致军团病,这是一种可能致命的呼吸道感染。疾病病原学可能涉及军团菌在原生生物细胞内的复制,本研究旨在描述军团菌与原生生物的关系,以开发新的爆发预防靶点。在6个月的时间里,研究人员从南卡罗来纳州的一个水冷塔收集了水和沉积物样本。同时,记录了多个环境参数。利用16S rRNA基因V4区和252bp的18S rRNA基因片段对细菌和真核生物群落进行了鉴定。通过共现网络分析来阐明军团菌与原生生物随时间的相关性。我们发现军团菌随着季节的推移与不同的原生生物相关。棘阿米巴在早春与军团菌相关,其次是晚春和初夏的万ella和Korotnevella,在仲夏与棘阿米巴相关。已知万尼拉和棘阿米巴是军团菌的潜在宿主,而Korotnevella是一种潜在的未记录宿主。在环境参数中,温度与原生生物属有较强的相关性,表明军团菌丰度受温度依赖性原生生物可用性的驱动。我们的研究结果强调了与军团菌水平升高相关的生态变化,这为帮助预测和预防疾病爆发提供了潜在的目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Environmental Microbiology Reports
Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY
CiteScore
6.00
自引率
3.00%
发文量
91
审稿时长
3.0 months
期刊介绍: The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信