Sweet Science: Exploring the Impact of Fructose and Glucose on Brown Adipocyte Differentiation Using Optical Diffraction Tomography

IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Pooja Anantha, Xiangdong Wu, Salaheldeen Elsaid, Piyush Raj, Junkai Hu, Ishan Barman, Sui Seng Tee
{"title":"Sweet Science: Exploring the Impact of Fructose and Glucose on Brown Adipocyte Differentiation Using Optical Diffraction Tomography","authors":"Pooja Anantha,&nbsp;Xiangdong Wu,&nbsp;Salaheldeen Elsaid,&nbsp;Piyush Raj,&nbsp;Junkai Hu,&nbsp;Ishan Barman,&nbsp;Sui Seng Tee","doi":"10.1002/jcb.70052","DOIUrl":null,"url":null,"abstract":"<p>The thermogenic capacity of brown adipose tissue (BAT) has garnered much attention for its potential to regulate systemic energy balance. BAT depot size and function need to be tightly regulated to prevent loss of metabolic homeostasis due to energy dissipation via non-shivering thermogenesis. While adipocyte-intrinsic mechanisms controlling thermogenesis are critical, an increasing appreciation for the role of the BAT microenvironment is emerging. For example, changes in circulating hexoses due to dietary intake have shown to impact BAT function. Here, we show that murine BAT preadipocytes metabolism is impacted when fructose is used as the sole carbon source. Similarly, differentiation medium containing only fructose yield mature adipocytes with fewer lipid droplets, with a concomitant decrease in adipogenic genes. These deficiencies are also observed in human BAT preadipocytes, where cutting-edge optical imaging modalities show a decrease in total cell mass and lipid mass in fructose-only medium. Taken together, the metabolic microenvironment significantly impacts BAT growth and function, with implications for the role of diets potentially mitigating the efficacy of BAT-targeted therapies.</p>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 6","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcb.70052","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of cellular biochemistry","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jcb.70052","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The thermogenic capacity of brown adipose tissue (BAT) has garnered much attention for its potential to regulate systemic energy balance. BAT depot size and function need to be tightly regulated to prevent loss of metabolic homeostasis due to energy dissipation via non-shivering thermogenesis. While adipocyte-intrinsic mechanisms controlling thermogenesis are critical, an increasing appreciation for the role of the BAT microenvironment is emerging. For example, changes in circulating hexoses due to dietary intake have shown to impact BAT function. Here, we show that murine BAT preadipocytes metabolism is impacted when fructose is used as the sole carbon source. Similarly, differentiation medium containing only fructose yield mature adipocytes with fewer lipid droplets, with a concomitant decrease in adipogenic genes. These deficiencies are also observed in human BAT preadipocytes, where cutting-edge optical imaging modalities show a decrease in total cell mass and lipid mass in fructose-only medium. Taken together, the metabolic microenvironment significantly impacts BAT growth and function, with implications for the role of diets potentially mitigating the efficacy of BAT-targeted therapies.

Abstract Image

甜科学:利用光学衍射断层扫描探索果糖和葡萄糖对棕色脂肪细胞分化的影响
棕色脂肪组织(BAT)的产热能力因其调节全身能量平衡的潜力而受到广泛关注。BAT库的大小和功能需要严格调节,以防止由于非寒颤产热能量耗散而导致代谢稳态的丧失。虽然脂肪细胞控制产热的内在机制是至关重要的,但对BAT微环境的作用的认识正在增加。例如,饮食摄入引起的循环己糖的变化已显示会影响BAT功能。在这里,我们发现当果糖作为唯一的碳源时,小鼠BAT前脂肪细胞的代谢受到影响。同样,仅含果糖的分化培养基产生成熟的脂肪细胞,脂滴较少,同时脂肪生成基因减少。在人类BAT前脂肪细胞中也观察到这些缺陷,其中尖端光学成像模式显示,在仅含果糖的培养基中,总细胞质量和脂质质量减少。综上所述,代谢微环境显著影响BAT的生长和功能,这意味着饮食的作用可能会降低BAT靶向治疗的疗效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of cellular biochemistry
Journal of cellular biochemistry 生物-生化与分子生物学
CiteScore
9.90
自引率
0.00%
发文量
164
审稿时长
1 months
期刊介绍: The Journal of Cellular Biochemistry publishes descriptions of original research in which complex cellular, pathogenic, clinical, or animal model systems are studied by biochemical, molecular, genetic, epigenetic or quantitative ultrastructural approaches. Submission of papers reporting genomic, proteomic, bioinformatics and systems biology approaches to identify and characterize parameters of biological control in a cellular context are encouraged. The areas covered include, but are not restricted to, conditions, agents, regulatory networks, or differentiation states that influence structure, cell cycle & growth control, structure-function relationships.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信