Tenofovir exerts antiviral effects in pregnant women with chronic hepatitis B by influencing the expression of miR-21-5p

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research Pub Date : 2025-06-30 DOI:10.1111/jog.16355

Lei Zhang, Juan Du, Lan Shao, Cuili Yang, Yu Wang, Zhongfu Mo, Jinyu Gu

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引用次数: 0

Abstract

Objective

In pregnant women with chronic hepatitis B (CHB), tenofovir (TDF) has demonstrated significant efficacy in disrupting the replication of the hepatitis B virus (HBV) and in preventing mother-to-child transmission (MTCT). This study seeks to elucidate the role of miR-21-5p in mediating the antiviral effect of TDF.

Methods

Initially, the levels of miR-21-5p in pregnant women with and without CHB were compared by RT-qPCR assay. Subsequently, a comparison was made between the levels of HBV and miR-21-5p prior to and following treatment with TDF. Moreover, the correlation between miR-21-5p levels and HBV DNA, HBsAg or HBeAg was analyzed by Pearson correlation analysis. Lastly, HBV-infected huh-7 cells were cultured in vitro. Then, RT-qPCR and ELISA assays were conducted to investigate the influence of modulating miR-21-5p levels on HBV replication.

Results

miR-21-5p levels were significantly increased in CHB pregnant women. Following treatment with TDF, HBV was effectively controlled. More crucially, TDF led to a reduction in miR-21-5p levels. The level of miR-21-5p exhibited a positive correlation with HBV DNA, HBsAg and HBeAg. In cell experiments, the inhibition of miR-21-5p resulted in a significant decrease in levels of HBV DNA, HBsAg, and HBeAg. Conversely, overexpression of miR-21-5p yielded opposing results.

Conclusion

In pregnant women with CHB, TDF may inhibit HBV replication by inhibiting the expression of miR-21-5p. This mechanism contributes to the mitigation of infection exacerbation.

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替诺福韦通过影响miR-21-5p的表达在慢性乙型肝炎孕妇中发挥抗病毒作用

在患有慢性乙型肝炎（CHB）的孕妇中，替诺福韦（TDF）在破坏乙型肝炎病毒（HBV）的复制和预防母婴传播（MTCT）方面显示出显著的疗效。本研究旨在阐明miR-21-5p在介导TDF抗病毒作用中的作用。方法首先采用RT-qPCR法比较合并和未合并CHB的孕妇miR-21-5p的水平。随后，比较了TDF治疗前后HBV和miR-21-5p的水平。采用Pearson相关分析分析miR-21-5p水平与HBV DNA、HBsAg或HBeAg的相关性。最后，体外培养hbv感染的huh-7细胞。然后，通过RT-qPCR和ELISA检测，研究调节miR-21-5p水平对HBV复制的影响。结果miR-21-5p水平在CHB孕妇中显著升高。经TDF治疗后，HBV得到有效控制。更重要的是，TDF导致miR-21-5p水平降低。miR-21-5p水平与HBV DNA、HBsAg、HBeAg呈正相关。在细胞实验中，抑制miR-21-5p导致HBV DNA、HBsAg和HBeAg水平显著降低。相反，过表达miR-21-5p会产生相反的结果。结论在CHB孕妇中，TDF可能通过抑制miR-21-5p的表达来抑制HBV复制。这一机制有助于减轻感染恶化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Obstetrics and Gynaecology Research 医学-妇产科学

CiteScore

3.10

自引率

0.00%

发文量

376

审稿时长

3-6 weeks

期刊介绍： The Journal of Obstetrics and Gynaecology Research is the official Journal of the Asia and Oceania Federation of Obstetrics and Gynecology and of the Japan Society of Obstetrics and Gynecology, and aims to provide a medium for the publication of articles in the fields of obstetrics and gynecology. The Journal publishes original research articles, case reports, review articles and letters to the editor. The Journal will give publication priority to original research articles over case reports. Accepted papers become the exclusive licence of the Journal. Manuscripts are peer reviewed by at least two referees and/or Associate Editors expert in the field of the submitted paper.