A mitochondria-targeted fluorescent probe toward cysteine in drug-induced liver injury models

IF 4.1 3区工程技术 Q2 CHEMISTRY, APPLIED

Dyes and Pigments Pub Date : 2025-06-27 DOI:10.1016/j.dyepig.2025.113004

Ying Shi , Wenjing Li , Qingyuan Yi , Yiping Wang , Zhibo Huang , Mian Wang , Jianyi Wang

引用次数: 0

Abstract

In this study, we developed a mitochondria-targeting fluorescent probe SY-Cys toward Cys based on a hemicyanine scaffold. SY-Cys features rapid response (4 min), high sensitivity (LOD = 0.16 μM), good selectivity without interference from common biomarkers. The responsive mechanism of SY-Cys towards Cys is an intramolecular cyclization-ICT mechanism. SY-Cys visualized oxidative stress-driven Cys upregulation during APAP-induced hepatotoxicity in HepG2 cells and NAC-mediated metabolic recovery. Furthermore, SY-Cys enabled in vivo imaging of Cys in DILI mice, corroborating cellular-level observations. Collectively, SY-Cys is powerful in monitoring Cys and is conducive to the early diagnosis of drug-induced liver injury diseases.

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线粒体靶向半胱氨酸荧光探针在药物性肝损伤模型中的应用

在这项研究中，我们开发了一种基于半花青碱支架的线粒体靶向荧光探针SY-Cys。SY-Cys具有响应速度快（4 min）、灵敏度高（LOD = 0.16 μM）、选择性好、不受常见生物标志物干扰等特点。SY-Cys对Cys的响应机制是分子内环化- ict机制。在apap诱导的HepG2细胞肝毒性和nac介导的代谢恢复过程中，y -Cys可见氧化应激驱动的Cys上调。此外，SY-Cys在DILI小鼠体内成像，证实了细胞水平的观察结果。综上所述，y -Cys在监测Cys方面具有强大的功能，有利于药物性肝损伤疾病的早期诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.