Genetic markers at the crossroads: toll-like receptor polymorphisms and Plasmodium vivax malaria parasitological aspects from the Brazil-French Guiana border.

IF 1.9 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-06-20 DOI:10.1093/trstmh/traf061

Marcelo Cerilo-Filho, Aína D R Ramirez, Vanessa S Barbosa, Renata B Correa, Myrela C S de Jesus, Marrara P Sampaio, Leonardo A Miceli, Nathália F Reis, Rubens A O Menezes, Margarete S M Gomes, Tamirys S Pimenta, Gustavo C Cassiano, José R S Silva, Luciane M Storti-Melo, Andrea R S Baptista, Ricardo L D Machado

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引用次数: 0

Abstract

Background: Studies relating allelic variants in toll-like receptor (TLR) genes to the parasitological profile of Plasmodium vivax malaria are scarce. Therefore, we sought to assess whether polymorphisms in these genes can influence the clinical and parasitological aspects of individuals with P. vivax malaria in an endemic area from the Brazil-French Guiana border.

Methods: We analyzed 76 patients with P. vivax malaria. Parasitological parameters were collected, and genomic DNA was extracted from whole blood. The single nucleotide polymorphisms (SNPs) of TLR4 (rs4986790, rs4986791), TLR6 (rs5743810) and TLR9 genes (rs187084, rs5743836) were genotyped by qPCR. Association between the levels of parasitemia and gametocytes with the SNPs was performed using Kruskal-Wallis and Mann-Whitney tests. Parasitemia and gametocyte levels were adjusted for the polymorphisms using a linear regression model.

Results: There was a correlation between the TLR4 rs4986790, TLR6 rs5743810 and TLR9 rs187084 SNPs and parasitological aspects.

Conclusions: The results described here suggest that these genes' polymorphisms may have a role in the development of the inflammatory response during P. vivax malaria. The potential implications of these findings are discussed.

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十字路口的遗传标记：来自巴西-法属圭亚那边境的toll样受体多态性和间日疟原虫疟疾寄生虫学方面。

背景：关于toll样受体（TLR）基因等位变异与间日疟寄生虫学特征的研究很少。因此，我们试图评估这些基因的多态性是否会影响巴西-法属圭亚那边境流行地区间日疟原虫疟疾患者的临床和寄生虫学方面。方法：对76例间日疟患者进行分析。采集寄生虫学参数，提取全血基因组DNA。对TLR4基因（rs4986790、rs4986791）、TLR6基因（rss5743810）和TLR9基因（rs187084、rss5743836）的单核苷酸多态性进行qPCR分型。利用Kruskal-Wallis和Mann-Whitney试验，研究了寄生虫血症和配子细胞水平与snp之间的关系。利用线性回归模型对寄生血症和配子体水平进行多态性调整。结果：TLR4 rs4986790、TLR6 rss5743810和TLR9 rs187084 snp位点与寄生虫学方面存在相关性。结论：本文所描述的结果表明，这些基因的多态性可能在间日疟原虫疟疾期间炎症反应的发展中起作用。讨论了这些发现的潜在含义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transactions of The Royal Society of Tropical Medicine and Hygiene 医学-公共卫生、环境卫生与职业卫生

CiteScore

4.00

自引率

9.10%

发文量

115

审稿时长

4-8 weeks

期刊介绍： Transactions of the Royal Society of Tropical Medicine and Hygiene publishes authoritative and impactful original, peer-reviewed articles and reviews on all aspects of tropical medicine.