Feedback transcription regulation between OsNAC3 and OsDREB1A promotes postgermination growth in rice.

IF 11.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Chengwei Huang, Yongqi He, Wenwen Li, Ling Liu, Zepeng Wu, Yipeng Zhong, Chengjing Wang, Liling Peng, Shan Sun, Zhibo Huang, Jia Zhao, Zhoufei Wang
{"title":"Feedback transcription regulation between OsNAC3 and OsDREB1A promotes postgermination growth in rice.","authors":"Chengwei Huang, Yongqi He, Wenwen Li, Ling Liu, Zepeng Wu, Yipeng Zhong, Chengjing Wang, Liling Peng, Shan Sun, Zhibo Huang, Jia Zhao, Zhoufei Wang","doi":"10.1126/sciadv.adw0421","DOIUrl":null,"url":null,"abstract":"<p><p>Early postgermination growth is critical for the success of direct seeding in rice cultivation. However, the underlying mechanism determining postgermination growth of rice is largely unknown. Here, we report the rice OsDREB1A-OsNAC3-OsGA20ox1 transcriptional module that positively regulates the early postgermination growth through the gibberellic acid (GA) pathway. Transcription factor OsDREB1A improves postgermination growth by OsNAC3-mediated increase of GA biosynthesis. OsNAC3 can directly bind to the promoter of GA biosynthesis gene <i>OsGA20ox1</i>, which consequently activates its expression and increases GA content during the early postgermination growth stage. Furthermore, the expressions of <i>OsDREB1A</i> and <i>OsNAC3</i> are positively regulated by each other, and the cis-regulatory polymorphisms in promoters fine-tune their corresponding expressions to determine the natural variations of postgermination growth. The transferred elite haplotypes of <i>OsNAC3</i> and <i>OsDREB1A</i> and overexpression of both genes in <i>japonica</i> varieties contribute postgermination growth. The application of OsDREB1A and OsNAC3 is useful for the development of rice varieties for direct seeding cultivation.</p>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 26","pages":"eadw0421"},"PeriodicalIF":11.7000,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12204167/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Science Advances","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1126/sciadv.adw0421","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Early postgermination growth is critical for the success of direct seeding in rice cultivation. However, the underlying mechanism determining postgermination growth of rice is largely unknown. Here, we report the rice OsDREB1A-OsNAC3-OsGA20ox1 transcriptional module that positively regulates the early postgermination growth through the gibberellic acid (GA) pathway. Transcription factor OsDREB1A improves postgermination growth by OsNAC3-mediated increase of GA biosynthesis. OsNAC3 can directly bind to the promoter of GA biosynthesis gene OsGA20ox1, which consequently activates its expression and increases GA content during the early postgermination growth stage. Furthermore, the expressions of OsDREB1A and OsNAC3 are positively regulated by each other, and the cis-regulatory polymorphisms in promoters fine-tune their corresponding expressions to determine the natural variations of postgermination growth. The transferred elite haplotypes of OsNAC3 and OsDREB1A and overexpression of both genes in japonica varieties contribute postgermination growth. The application of OsDREB1A and OsNAC3 is useful for the development of rice varieties for direct seeding cultivation.

OsNAC3和OsDREB1A之间的反馈转录调控促进水稻萌发后生长。
发芽后早期生长是水稻直播栽培成功的关键。然而,决定水稻发芽后生长的潜在机制在很大程度上是未知的。在这里,我们报道了水稻OsDREB1A-OsNAC3-OsGA20ox1转录模块,该模块通过赤霉素(GA)途径正向调节萌发后早期生长。转录因子OsDREB1A通过osnac3介导的GA生物合成增加促进萌发后生长。OsNAC3可以直接结合GA生物合成基因OsGA20ox1的启动子,激活其表达,在萌发后早期生长阶段增加GA含量。此外,OsDREB1A和OsNAC3的表达相互正向调节,启动子的顺式调控多态性微调了它们对应的表达,决定了萌发后生长的自然变化。OsNAC3和OsDREB1A的优秀单倍型的转移以及这两个基因在粳稻品种中的过表达有助于萌发后生长。OsDREB1A和OsNAC3的应用对水稻直播栽培品种的选育具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Science Advances
Science Advances 综合性期刊-综合性期刊
CiteScore
21.40
自引率
1.50%
发文量
1937
审稿时长
29 weeks
期刊介绍: Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信