{"title":"Feedback transcription regulation between OsNAC3 and OsDREB1A promotes postgermination growth in rice.","authors":"Chengwei Huang, Yongqi He, Wenwen Li, Ling Liu, Zepeng Wu, Yipeng Zhong, Chengjing Wang, Liling Peng, Shan Sun, Zhibo Huang, Jia Zhao, Zhoufei Wang","doi":"10.1126/sciadv.adw0421","DOIUrl":null,"url":null,"abstract":"<p><p>Early postgermination growth is critical for the success of direct seeding in rice cultivation. However, the underlying mechanism determining postgermination growth of rice is largely unknown. Here, we report the rice OsDREB1A-OsNAC3-OsGA20ox1 transcriptional module that positively regulates the early postgermination growth through the gibberellic acid (GA) pathway. Transcription factor OsDREB1A improves postgermination growth by OsNAC3-mediated increase of GA biosynthesis. OsNAC3 can directly bind to the promoter of GA biosynthesis gene <i>OsGA20ox1</i>, which consequently activates its expression and increases GA content during the early postgermination growth stage. Furthermore, the expressions of <i>OsDREB1A</i> and <i>OsNAC3</i> are positively regulated by each other, and the cis-regulatory polymorphisms in promoters fine-tune their corresponding expressions to determine the natural variations of postgermination growth. The transferred elite haplotypes of <i>OsNAC3</i> and <i>OsDREB1A</i> and overexpression of both genes in <i>japonica</i> varieties contribute postgermination growth. The application of OsDREB1A and OsNAC3 is useful for the development of rice varieties for direct seeding cultivation.</p>","PeriodicalId":21609,"journal":{"name":"Science Advances","volume":"11 26","pages":"eadw0421"},"PeriodicalIF":11.7000,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12204167/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Science Advances","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1126/sciadv.adw0421","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Early postgermination growth is critical for the success of direct seeding in rice cultivation. However, the underlying mechanism determining postgermination growth of rice is largely unknown. Here, we report the rice OsDREB1A-OsNAC3-OsGA20ox1 transcriptional module that positively regulates the early postgermination growth through the gibberellic acid (GA) pathway. Transcription factor OsDREB1A improves postgermination growth by OsNAC3-mediated increase of GA biosynthesis. OsNAC3 can directly bind to the promoter of GA biosynthesis gene OsGA20ox1, which consequently activates its expression and increases GA content during the early postgermination growth stage. Furthermore, the expressions of OsDREB1A and OsNAC3 are positively regulated by each other, and the cis-regulatory polymorphisms in promoters fine-tune their corresponding expressions to determine the natural variations of postgermination growth. The transferred elite haplotypes of OsNAC3 and OsDREB1A and overexpression of both genes in japonica varieties contribute postgermination growth. The application of OsDREB1A and OsNAC3 is useful for the development of rice varieties for direct seeding cultivation.
期刊介绍:
Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.