Effects of chemotherapy on skeletal muscle mitochondrial oxidative capacity using near-infrared spectroscopy (NIRS): Protocol paper for an observational mixed model repeated measures design in patients with breast and gynecological cancer.

IF 2.6 3区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
PLoS ONE Pub Date : 2025-06-27 eCollection Date: 2025-01-01 DOI:10.1371/journal.pone.0315351
Randolph Edward Hutchison, Shannon Smith, Chloe Caudell, Sara Biddle, William Larry Gluck, Jennifer L Trilk
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引用次数: 0

Abstract

Mitochondrial dysfunction, a hallmark of metabolic disturbances in the skeletal muscle, has previously been studied in health participants using invasive muscle biopsy and/or time consuming, high-cost magnetic resonance spectroscopy. However, less is understood regarding mitochondrial dysfunction in patients with cancer. Near infrared spectroscopy (NIRS) is a non-invasive and cost-effective approach to assessing mitochondrial function of skeletal muscle by measuring oxygenated and deoxygenated hemoglobin and calculating the resulting tissue saturation index. NIRS has not yet been utilized to evaluate skeletal muscle change in cancer patients throughout chemotherapy. Therefore, we plan to conduct a single center clinical trial, using an observational mixed model repeated measures design to evaluate the change in mitochondrial oxidative capacity from baseline and throughout progression of an oncologist-prescribed chemotherapy regimen. Evaluation of mitochondrial function will be performed by taking real-time, NIRS in-situ measurements within the working muscle during stationary cycling exercise and subsequent recovery periods (e.g., "on" kinetics and "off" kinetics). We also plan to observe if there is a difference in mitochondrial oxidative capacity between different chemotherapy regimens across different patients. This pilot study will provide information on the feasibility of capturing on/off kinetics mitochondrial function data longitudinally using NIRS in patients newly diagnosed with breast or gynecological cancer, provide preliminary data for future extramural funding, as well as inform the scientific community of results through dissemination via conferences and peer-reviewed journal publications. This clinical trial has been registered with clinicaltrials.gov (Identifier: NCT006672497). Data collection started on July 26, 2021 and is ongoing through March 27, 2026. The authors confirm that all ongoing and related trials for this observation trial (no drug or intervention) are registered.

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化疗对骨骼肌线粒体氧化能力的影响使用近红外光谱(NIRS):观察性混合模型重复测量设计的方案文件,用于乳腺癌和妇科癌症患者。
线粒体功能障碍是骨骼肌代谢紊乱的标志,此前曾在健康参与者中使用侵入性肌肉活检和/或耗时、高成本的磁共振波谱进行过研究。然而,人们对癌症患者的线粒体功能障碍知之甚少。近红外光谱(NIRS)是通过测量含氧和脱氧血红蛋白并计算由此产生的组织饱和指数来评估骨骼肌线粒体功能的一种无创且经济高效的方法。近红外光谱尚未用于评估癌症患者在化疗期间骨骼肌的变化。因此,我们计划进行一项单中心临床试验,使用观察性混合模型重复测量设计来评估线粒体氧化能力从基线到肿瘤医生规定的化疗方案的整个过程中的变化。线粒体功能的评估将通过在固定循环运动和随后的恢复期间(例如,“开”动力学和“关”动力学)对工作肌肉进行实时、近红外光谱原位测量来进行。我们还计划观察不同患者的不同化疗方案之间线粒体氧化能力是否存在差异。这项试点研究将提供关于在新诊断为乳腺癌或妇科癌症的患者中使用近红外光谱纵向捕获开/关动力学线粒体功能数据的可行性信息,为未来的校外资助提供初步数据,并通过会议和同行评审的期刊出版物将结果告知科学界。该临床试验已在clinicaltrials.gov注册(标识符:NCT006672497)。数据收集于2021年7月26日开始,一直持续到2026年3月27日。作者确认,所有正在进行的和相关的观察试验(无药物或干预)均已注册。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
PLoS ONE
PLoS ONE 生物-生物学
CiteScore
6.20
自引率
5.40%
发文量
14242
审稿时长
3.7 months
期刊介绍: PLOS ONE is an international, peer-reviewed, open-access, online publication. PLOS ONE welcomes reports on primary research from any scientific discipline. It provides: * Open-access—freely accessible online, authors retain copyright * Fast publication times * Peer review by expert, practicing researchers * Post-publication tools to indicate quality and impact * Community-based dialogue on articles * Worldwide media coverage
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