{"title":"Super-resolution microscopy for structural biology.","authors":"John S H Danial","doi":"10.1038/s41592-025-02731-1","DOIUrl":null,"url":null,"abstract":"<p><p>Super-resolution microscopy (SRM) has revolutionized biological imaging, enabling the visualization of biological structures at molecular resolution. However, the diversity of SRM techniques, each operating through different mechanisms, combined with the lack of a clear consensus on the definition of resolution, has hindered the biological community from fully recognizing SRM's contribution to structural biology. I propose a framework for defining, measuring and reporting resolution in SRM. Within this framework, I examine the capabilities of state-of-the-art methods that can achieve 'ångström-scale resolution' and clarify the level of structural detail users can expect to observe. Finally, drawing from recent advancements, I explore pathways to extend SRM towards live structural imaging.</p>","PeriodicalId":18981,"journal":{"name":"Nature Methods","volume":" ","pages":""},"PeriodicalIF":36.1000,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Methods","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1038/s41592-025-02731-1","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Super-resolution microscopy (SRM) has revolutionized biological imaging, enabling the visualization of biological structures at molecular resolution. However, the diversity of SRM techniques, each operating through different mechanisms, combined with the lack of a clear consensus on the definition of resolution, has hindered the biological community from fully recognizing SRM's contribution to structural biology. I propose a framework for defining, measuring and reporting resolution in SRM. Within this framework, I examine the capabilities of state-of-the-art methods that can achieve 'ångström-scale resolution' and clarify the level of structural detail users can expect to observe. Finally, drawing from recent advancements, I explore pathways to extend SRM towards live structural imaging.
期刊介绍：
Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.
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