Detection of alpha-HPV types in cutaneous squamous cell carcinoma associated with markers of carcinogenesis: a retrospective study.

IF 3.7 4区医学 Q1 DERMATOLOGY

Clinical and Experimental Dermatology Pub Date : 2025-06-20 DOI:10.1093/ced/llaf264

Florence Brunet-Possenti, Morgane Weiss, Jean-Luc Prétet, Lydia Deschamps, Vincent Descamps, Caroline Halimi, Diane Descamps, Jade Ghosn, Romane Guilbaud, Charlotte Charpentier, Valentine Marie Ferré

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引用次数: 0

Abstract

Background: While the role of oncogenic alpha (α)-HPVs in anogenital and oral mucosal cancers is well-established, their involvement in skin carcinogenesis remains unexplored.

Objective: To investigate the role of α-HPV in cutaneous squamous cell carcinoma (cSCC).

Methods: This retrospective study included 129 formalin-fixed, paraffin-embedded cSCC samples. The main outcomes were the proportion of α-HPV positive cSCC and the characterization of viral features, assessed through HPV typing and viral DNA integration analysis.

Results: The study included 99 cases of invasive cSCCs and 30 Bowen's disease. HPV DNA was detected in 56.6% of samples. HPV from the α genus were identified in 25 samples, (19.4%), with only a single α-HPV type detected in each positive sample. HPV16 was the main type, detected in 10 samples. Peripheral healthy skin tested negative in 10 out of 11 α-HPV-positive samples. Viral DNA was integrated in host-cell DNA in 8 out of 9 HPV16-positive samples.

Conclusions: This study highlights a significant prevalence of α-HPV-positive samples among both invasive and in situ cSCC. The virological findings support a carcinogenic role of α-HPV in cSCC.

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在与癌变标志物相关的皮肤鳞状细胞癌中检测α - hpv类型：一项回顾性研究。

背景：虽然致癌的α (α)- hpv在肛门生殖器癌和口腔粘膜癌中的作用已经确立，但它们在皮肤癌变中的作用仍未被探索。目的：探讨α-HPV在皮肤鳞状细胞癌（cSCC）中的作用。方法：回顾性研究129例福尔马林固定石蜡包埋cSCC标本。主要结果是α-HPV阳性cSCC的比例和病毒特征的表征，通过HPV分型和病毒DNA整合分析评估。结果：本研究纳入浸润性cSCCs 99例，Bowen病30例。56.6%的样本中检出HPV DNA。25份样本中检出α属HPV，占19.4%，每个阳性样本中仅检出单一α-HPV型。HPV16型为主要类型，共检出10份。在11个α- hpv阳性样本中，10个外周健康皮肤检测为阴性。病毒DNA在9个hpv16阳性样本中有8个整合到宿主细胞DNA中。结论：本研究强调了α- hpv阳性样本在侵袭性和原位cSCC中的显著患病率。病毒学研究结果支持α-HPV在cSCC中的致癌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Dermatology 医学-皮肤病学

CiteScore

3.20

自引率

2.40%

发文量

389

审稿时长

3-8 weeks

期刊介绍： Clinical and Experimental Dermatology (CED) is a unique provider of relevant and educational material for practising clinicians and dermatological researchers. We support continuing professional development (CPD) of dermatology specialists to advance the understanding, management and treatment of skin disease in order to improve patient outcomes.