Transcriptome responses of Anisopteromalus calandrae (Hymenoptera: Pteromalidae) females to sublethal deltamethrin exposure

IF 2.7 2区农林科学 Q1 ENTOMOLOGY

Journal of Stored Products Research Pub Date : 2025-06-29 DOI:10.1016/j.jspr.2025.102744

Weiwei Liu , Jianhua Lü , Chunqi Bai , Yafei Guo , Jiaqin Xi , Chao Guo

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引用次数: 0

Abstract

Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae) is a dominant parasitoid of stored grain pests. Deltamethrin is widely used for controlling stored grain pests. However, deltamethrin also has a certain potential negative impact on parasitoids due to its broad-spectrum insecticidal property, which poses a challenge for the scientific and efficient collaborative application of biological and chemical control. Thus, the toxicity of deltamethrin on female A. calandrae was investigated when exposed to 0.00, 0.02, 0.04, and 0.08 μg/cm² for 4 d (the corresponding female A. calandrae samples were referred as DM0, DM0.02, DM0.04, and DM0.08, respectively) and transcriptomic analysis was conducted to reveal their patterns of response. Compared to control (11 d), the total lethal times of A. calandrae females exposed to 0.02, 0.04, 0.06, 0.08, and 0.10 μg/cm² of deltamethrin was 8, 7, 7, 5, and 4 d, respectively. Transcriptomic data revealed 3040, 5377, and 4517 differentially expressed genes (DEGs) in DM0.02, DM0.04, and DM0.08 groups, compared with the control (DM0), respectively. GO and KEGG analyses indicated that pathways involved in longevity regulation, cuticle biosynthesis, fatty acid metabolism, and detoxification (cytochrome P450) were affected. WGCNA revealed a highly correlated module (turquoise) containing key resistance-related genes, such as TRINITY_DN882_c0_g1_i21. The current results are in favor of improving the compatibility of biological and chemical pest control strategies in grain storage systems.

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膜翅目：凤蝶科雌蜂对亚致死溴氰菊酯暴露的转录组反应

万年蜂（膜翅目：万年蜂科）是储粮害虫的优势寄生蜂。溴氰菊酯广泛用于储粮害虫的防治。然而，溴氰菊酯具有广谱杀虫特性，对拟寄生虫也有一定的潜在负面影响，这给生物与化学防治的科学高效协同应用带来了挑战。为此，研究了溴氰菊酯分别为0.00、0.02、0.04和0.08 μg/cm2（对应的万桔甲雌虫样本分别为DM0、DM0.02、DM0.04和DM0.08） 4 d对万桔甲雌虫的毒力，并通过转录组学分析揭示了它们的反应规律。与对照（11 d）相比，溴氰菊酯浓度为0.02、0.04、0.06、0.08和0.10 μg/cm2时，月季甲蚜雌虫的总致死时间分别为8、7、7、5和4 d。转录组学数据显示，与对照组（DM0）相比，DM0.02、DM0.04和DM0.08组分别有3040、5377和4517个差异表达基因（deg）。GO和KEGG分析表明，参与寿命调节、角质层生物合成、脂肪酸代谢和解毒（细胞色素P450）的途径受到影响。WGCNA发现了一个高度相关的模块（绿松石），包含关键的抗性相关基因，如TRINITY_DN882_c0_g1_i21。目前的研究结果有利于提高粮食储存系统中生物和化学防治策略的相容性。

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来源期刊

Journal of Stored Products Research 生物-昆虫学

CiteScore

5.70

自引率

18.50%

发文量

112

审稿时长

45 days

期刊介绍： The Journal of Stored Products Research provides an international medium for the publication of both reviews and original results from laboratory and field studies on the preservation and safety of stored products, notably food stocks, covering storage-related problems from the producer through the supply chain to the consumer. Stored products are characterised by having relatively low moisture content and include raw and semi-processed foods, animal feedstuffs, and a range of other durable items, including materials such as clothing or museum artefacts.