Weiwei Liu , Jianhua Lü , Chunqi Bai , Yafei Guo , Jiaqin Xi , Chao Guo
{"title":"Transcriptome responses of Anisopteromalus calandrae (Hymenoptera: Pteromalidae) females to sublethal deltamethrin exposure","authors":"Weiwei Liu , Jianhua Lü , Chunqi Bai , Yafei Guo , Jiaqin Xi , Chao Guo","doi":"10.1016/j.jspr.2025.102744","DOIUrl":null,"url":null,"abstract":"<div><div><em>Anisopteromalus calandrae</em> (Howard) (Hymenoptera: Pteromalidae) is a dominant parasitoid of stored grain pests. Deltamethrin is widely used for controlling stored grain pests. However, deltamethrin also has a certain potential negative impact on parasitoids due to its broad-spectrum insecticidal property, which poses a challenge for the scientific and efficient collaborative application of biological and chemical control. Thus, the toxicity of deltamethrin on female <em>A. calandrae</em> was investigated when exposed to 0.00, 0.02, 0.04, and 0.08 μg/cm<sup>2</sup> for 4 d (the corresponding female <em>A. calandrae</em> samples were referred as DM0, DM0.02, DM0.04, and DM0.08, respectively) and transcriptomic analysis was conducted to reveal their patterns of response. Compared to control (11 d), the total lethal times of <em>A. calandrae</em> females exposed to 0.02, 0.04, 0.06, 0.08, and 0.10 μg/cm<sup>2</sup> of deltamethrin was 8, 7, 7, 5, and 4 d, respectively. Transcriptomic data revealed 3040, 5377, and 4517 differentially expressed genes (DEGs) in DM0.02, DM0.04, and DM0.08 groups, compared with the control (DM0), respectively. GO and KEGG analyses indicated that pathways involved in longevity regulation, cuticle biosynthesis, fatty acid metabolism, and detoxification (cytochrome P450) were affected. WGCNA revealed a highly correlated module (turquoise) containing key resistance-related genes, such as TRINITY_DN882_c0_g1_i21. The current results are in favor of improving the compatibility of biological and chemical pest control strategies in grain storage systems.</div></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":"114 ","pages":"Article 102744"},"PeriodicalIF":2.7000,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Stored Products Research","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022474X25002036","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae) is a dominant parasitoid of stored grain pests. Deltamethrin is widely used for controlling stored grain pests. However, deltamethrin also has a certain potential negative impact on parasitoids due to its broad-spectrum insecticidal property, which poses a challenge for the scientific and efficient collaborative application of biological and chemical control. Thus, the toxicity of deltamethrin on female A. calandrae was investigated when exposed to 0.00, 0.02, 0.04, and 0.08 μg/cm2 for 4 d (the corresponding female A. calandrae samples were referred as DM0, DM0.02, DM0.04, and DM0.08, respectively) and transcriptomic analysis was conducted to reveal their patterns of response. Compared to control (11 d), the total lethal times of A. calandrae females exposed to 0.02, 0.04, 0.06, 0.08, and 0.10 μg/cm2 of deltamethrin was 8, 7, 7, 5, and 4 d, respectively. Transcriptomic data revealed 3040, 5377, and 4517 differentially expressed genes (DEGs) in DM0.02, DM0.04, and DM0.08 groups, compared with the control (DM0), respectively. GO and KEGG analyses indicated that pathways involved in longevity regulation, cuticle biosynthesis, fatty acid metabolism, and detoxification (cytochrome P450) were affected. WGCNA revealed a highly correlated module (turquoise) containing key resistance-related genes, such as TRINITY_DN882_c0_g1_i21. The current results are in favor of improving the compatibility of biological and chemical pest control strategies in grain storage systems.
期刊介绍:
The Journal of Stored Products Research provides an international medium for the publication of both reviews and original results from laboratory and field studies on the preservation and safety of stored products, notably food stocks, covering storage-related problems from the producer through the supply chain to the consumer. Stored products are characterised by having relatively low moisture content and include raw and semi-processed foods, animal feedstuffs, and a range of other durable items, including materials such as clothing or museum artefacts.