Parishin from Gastrodia elata ameliorates DSS induced colitis and anxiety-like behavior in mice by regulating intestinal barrier function and microviota-gut-brain axis

IF 6.7 1区医学 Q1 CHEMISTRY, MEDICINAL

Phytomedicine Pub Date : 2025-06-22 DOI:10.1016/j.phymed.2025.157019

Xiaoxue Liu , Hejiang Zhou , Na Yang , Lu-Jun Yang , Zheng-Ying Zi , Ya-Lan Han , Jinsong He , Ling-Yan Su

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引用次数: 0

Abstract

Background

Inflammatory bowel diseases (IBDs), including colitis, are commonly associated with dysfunctional intestinal barriers and dysregulated gut microbiota. Moreover, current medications for IBD cause serious side effects with prolonged use. Parishin, a phenolic glucoside isolated from Gastrodia elata, is known for its medicinal value in anti-aging properties. However, the therapeutic effects of Parishin on colitis and the underlying mechanisms remain largely unexplored.

Purpose

The aim of this study was to examine the protective effects of Parishin on colitis and related anxiety mood disorder in mice, as well as to explore its potential mechanisms.

Methods

This study employed a DSS-induced colitis mouse model to evaluate Parishin’s therapeutic effects. Mice were divided into control, model, and Parishin treatment groups, followed by DAI, histopathology, inflammatory cytokines, gut barrier proteins, gene expression, behavioral changes, and gut microbiota composition.

Results

We demonstrated that Parishin alleviated symptoms such as weight loss, a colon shortening, and a high disease activity index in a dextran sulfate sodium (DSS)-induced colitis mouse model. Parishin strengthened the intestinal barrier by increasing the expression of tight junction proteins and mucin. Furthermore, Parishin significantly reduced the production of pro-inflammatory cytokines in the serum, colon and spleen of mice with DSS. Notably, Parishin treatment attenuated DSS-induced anxiety-like behavior by activating GABA receptors and regulating the FKBP5 level. Gut microbiota analysis showed that Parishin restored the DSS-disturbed microbiota of the mice.

Conclusion

These findings demonstrate that Parishin may attenuate DSS-induced colitis and anxiety-like behavior by enhancing the intestinal barrier and regulating the gut microbiota, supporting the development of a Parishin-based strategy for IBD prevention or treatment.

Abstract Image

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天麻Parishin通过调节肠道屏障功能和微肠-肠-脑轴改善DSS诱导小鼠结肠炎和焦虑样行为

背景炎性肠病（IBDs），包括结肠炎，通常与肠道屏障功能障碍和肠道微生物群失调有关。此外，目前的IBD药物长期使用会导致严重的副作用。Parishin是一种从天麻中分离出来的酚类葡萄糖苷，以其抗衰老的药用价值而闻名。然而，Parishin对结肠炎的治疗效果和潜在的机制在很大程度上仍未被探索。目的观察巴利辛对小鼠结肠炎及相关焦虑情绪障碍的保护作用，并探讨其可能的机制。方法采用dss诱导结肠炎小鼠模型，观察巴利辛的治疗效果。将小鼠分为对照组、模型组和Parishin治疗组，分别进行DAI、组织病理学、炎症因子、肠道屏障蛋白、基因表达、行为改变和肠道菌群组成的观察。结果在葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型中，Parishin减轻了体重减轻、结肠缩短和高疾病活动指数等症状。Parishin通过增加紧密连接蛋白和粘蛋白的表达来增强肠屏障。此外，Parishin显著降低了DSS小鼠血清、结肠和脾脏中促炎细胞因子的产生。值得注意的是，Parishin治疗通过激活GABA受体和调节FKBP5水平来减轻dss诱导的焦虑样行为。肠道菌群分析显示，Parishin恢复了受dss干扰的小鼠肠道菌群。结论这些发现表明Parishin可能通过增强肠道屏障和调节肠道微生物群来减轻dss诱导的结肠炎和焦虑样行为，支持Parishin为基础的IBD预防或治疗策略的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytomedicine 医学-药学

CiteScore

10.30

自引率

5.10%

发文量

670

审稿时长

91 days

期刊介绍： Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.