Cryopreserved Skin Transplantation in a Nonhuman Primate Model.

Meghan Hu, Rafaela Belloni, Joseph Ladowski, Davide Schiliro, Alessandro Martinino, Allison Schwalb, Zishen Li, Mingqing Song, Kyha Williams, Alton B Farris, Stuart Knechtle, Jean Kwun
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Abstract

Background: Skin transplantation is often used in nonhuman primate (NHP) transplant research for sensitization and to assess tolerance. However, the availability of donor animals for skin can be constrained after organ donation. In this study, we evaluated the feasibility of cryopreserving skin patches to address this issue.

Methods: A full-thickness dorsal skin section with a diameter of ∼3 cm was excised. Following defatting process, the skin graft was immersed in a skin-cryopreservation media and preserved in liquid nitrogen. Prior to transplantation, the frozen skin was rapidly thawed using a 37°C water bath and thoroughly washed with normal saline. Cryopreserved syngeneic skin transplantations were performed, monitored, and assessed histologically.

Results: Autologous skin grafts preserved in liquid nitrogen with prolonged storage time (>1 month) demonstrated successful engraftment in NHP recipients. The cryopreserved skin exhibited well-preserved epidermis and dermis, with minimal distinction compared to non-cryopreserved samples. In fully engrafted cryopreserved skin grafts, dermal fibrosis appeared slightly less distinct at 28 days after transplantation. These differences could be attributable to anatomical location of the samples.

Conclusion: The cryopreserved skin showed well-preserved normal skin histology before and after skin transplantation. Here, we show that cryopreserved NHP skin can be stored for a prolonged time and grafted in a delayed manner. Cryopreserved skin could serve as a source when a living donor NHP is unavailable, including for testing donor-specific tolerance in the absence of the organ donor.

非人灵长类动物模型的低温皮肤移植。
背景:皮肤移植常用于非人灵长类动物(NHP)的移植研究,用于致敏和评估耐受性。然而,在器官捐献后,供皮动物的可用性可能受到限制。在本研究中,我们评估了低温保存皮肤贴片解决这一问题的可行性。方法:切除直径约3cm的全层背侧皮肤切片。脱脂后,将移植物浸入皮肤冷冻保存介质中,并在液氮中保存。移植前,用37°C水浴快速解冻冷冻皮肤,并用生理盐水彻底清洗。进行冷冻保存的同种皮肤移植,监测和组织学评估。结果:自体皮肤移植物在液氮中保存较长时间(10 ~ 10个月),NHP受者植皮成功。与非冷冻保存的样品相比,冷冻保存的皮肤表现出保存良好的表皮和真皮层。在完全植入术的低温保存皮肤移植中,皮肤纤维化在移植后28天出现稍微不那么明显。这些差异可能归因于样本的解剖位置。结论:冷冻保存皮肤在皮肤移植前后表现出良好的正常皮肤组织结构。在这里,我们表明低温保存的NHP皮肤可以长时间保存并以延迟的方式移植。当无法获得活体供体NHP时,冷冻保存的皮肤可以作为来源,包括在器官供体缺席的情况下测试供体特异性耐受性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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