Meghan Hu , Rafaela Belloni , Joseph Ladowski , Davide Schiliro , Alessandro Martinino , Allison Schwalb , Zishen Li , Mingqing Song , Kyha Williams , Alton B. Farris , Stuart Knechtle , Jean Kwun
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引用次数: 0
Abstract
Background
Skin transplantation is often used in nonhuman primate (NHP) transplant research for sensitization and to assess tolerance. However, the availability of donor animals for skin can be constrained after organ donation. In this study, we evaluated the feasibility of cryopreserving skin patches to address this issue.
Methods
A full-thickness dorsal skin section with a diameter of ∼3 cm was excised. Following defatting process, the skin graft was immersed in a skin-cryopreservation media and preserved in liquid nitrogen. Prior to transplantation, the frozen skin was rapidly thawed using a 37°C water bath and thoroughly washed with normal saline. Cryopreserved syngeneic skin transplantations were performed, monitored, and assessed histologically.
Results
Autologous skin grafts preserved in liquid nitrogen with prolonged storage time (>1 month) demonstrated successful engraftment in NHP recipients. The cryopreserved skin exhibited well-preserved epidermis and dermis, with minimal distinction compared to non-cryopreserved samples. In fully engrafted cryopreserved skin grafts, dermal fibrosis appeared slightly less distinct at 28 days after transplantation. These differences could be attributable to anatomical location of the samples.
Conclusion
The cryopreserved skin showed well-preserved normal skin histology before and after skin transplantation. Here, we show that cryopreserved NHP skin can be stored for a prolonged time and grafted in a delayed manner. Cryopreserved skin could serve as a source when a living donor NHP is unavailable, including for testing donor-specific tolerance in the absence of the organ donor.
期刊介绍:
Transplantation Proceedings publishes several different categories of manuscripts, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication.
The first type of manuscripts consists of sets of papers providing an in-depth expression of the current state of the art in various rapidly developing components of world transplantation biology and medicine. These manuscripts emanate from congresses of the affiliated transplantation societies, from Symposia sponsored by the Societies, as well as special Conferences and Workshops covering related topics.
Transplantation Proceedings also publishes several special sections including publication of Clinical Transplantation Proceedings, being rapid original contributions of preclinical and clinical experiences. These manuscripts undergo review by members of the Editorial Board.
Original basic or clinical science articles, clinical trials and case studies can be submitted to the journal?s open access companion title Transplantation Reports.