Single-cell transcriptional landscapes of Aedes aegypti midgut and fat body after a bloodmeal.

IF 11.1 Q1 CELL BIOLOGY
Cell genomics Pub Date : 2025-08-13 Epub Date: 2025-06-25 DOI:10.1016/j.xgen.2025.100924
Thomas Vial, Hélène Lopez-Maestre, Elodie Couderc, Silvain Pinaud, Virginia Howick, Jewelna Akorli, Mara Lawniczak, Guillaume Marti, Sarah Hélène Merkling
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引用次数: 0

Abstract

Aedes aegypti mosquitoes transmit arboviruses that pose a growing global health threat. After a bloodmeal, mosquitoes experience complex physiological changes orchestrated by the midgut and fat body, beginning with digestion and culminating in egg production. Our study provides comprehensive midgut and fat-body cell atlases using single-cell RNA sequencing and metabolomics. Our analyses reveal highly diverse cell populations specialized in digestion, metabolism, immunity, and reproduction. The midgut primarily comprises enterocytes, enteroendocrine, and intestinal stem cells, while the fat body features trophocytes and oenocytes but also a substantial hemocyte population and a newly found fat-body-yolk cell population. Additionally, Phasi Charoen-like virus was detected in midgut cells 7 days post bloodmeal. These findings highlight the complexity of mosquito abdominal tissues and inform the development of refined vector-control strategies, focusing on specific cell populations and metabolic pathways essential for mosquito reproductive success.

吸血后埃及伊蚊中肠和脂肪体的单细胞转录景观。
埃及伊蚊传播的虫媒病毒对全球健康构成日益严重的威胁。在吸血之后,蚊子经历了由中肠和脂肪体精心安排的复杂生理变化,从消化开始,到产卵结束。我们的研究利用单细胞RNA测序和代谢组学提供了全面的中肠和脂肪体细胞图谱。我们的分析揭示了高度多样化的细胞群专门消化,代谢,免疫和繁殖。中肠主要包括肠细胞、肠内分泌细胞和肠干细胞,而脂肪体以滋养细胞和卵泡细胞为特征,但也有大量的血细胞群和新发现的脂肪体卵黄细胞群。此外,在吸血后7天的中肠细胞中检测到夏伦样期病毒。这些发现突出了蚊子腹部组织的复杂性,并为改进媒介控制策略提供了信息,重点关注蚊子繁殖成功所必需的特定细胞群和代谢途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
7.10
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