Cationic Polymer Micelles as Carriers of Bioactive Sesquiterpene Lactones from Inula Helenium L. for Effective Treatment of Bacterial Biofilms.

IF 4.9 3区 医学 Q1 PHARMACOLOGY & PHARMACY
Rumena Stancheva, Tsvetozara Damyanova, Tsvetelina Paunova-Krasteva, Ralitsa Veleva, Tanya Topouzova-Hristova, Viktoria Ivanova, Antoaneta Trendafilova, Ivaylo Dimitrov, Stanislav Rangelov, Emi Haladjova
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引用次数: 0

Abstract

Objectives: Nanosized polymeric micelles (PMs) with an average size of about 80 nm and moderately positive ζ potential, based on an amphiphilic poly(4-methyl-piperazin-1-yl)-propenone)-b-polylactide (PMPP-PLA) block copolymer, were prepared. They were used as platforms for the delivery of bioactive sesquiterpene lactones from Inula helenium L. root extract. Methods: The PMs were characterized with good encapsulation efficiency as a maximum value of 72% was reached at a polymer-to-extract mass ratio of 10:1. The loaded micelles exhibited good colloidal stability. An in vitro release was performed showing a burst release profile. The biocompatibility of the resulting PMs was confirmed by assessing their cytotoxic effect on human keratinocytes in vitro by colorimetric assay and flow cytometry. Results: The systems demonstrated the capability to reduce the biomass of pre-formed Gram-positive and Gram-negative bacterial biofilms. Conclusions: The obtained data clearly determine a trend for a strong combined effect between the PMs and the root extract, distinguishing them with an excellent anti-biofilm potential and prospects for future applications in medical practice.

阳离子聚合物胶束载体对菊属植物生物活性倍半萜内酯有效处理细菌生物膜的研究。
目的:以两亲性聚(4-甲基-哌嗪-1-酰基)-丙烯)-b-聚乳酸(PMPP-PLA)嵌段共聚物为基础,制备了平均尺寸约为80 nm、ζ电位中等的纳米级聚合物胶束(pm)。它们被用作Inula helenium L.根提取物中具有生物活性的倍半萜内酯的传递平台。方法:在聚合物与提取物质量比为10:1时,包封率最高可达72%。负载胶束表现出良好的胶体稳定性。体外释放表现为爆发释放。通过比色法和流式细胞术评估其体外对人角质形成细胞的细胞毒作用,证实了所制pmms的生物相容性。结果:该系统能够减少预先形成的革兰氏阳性和革兰氏阴性细菌生物膜的生物量。结论:所获得的数据清楚地确定了pm与根提取物之间的强联合作用趋势,区分出它们具有良好的抗生物膜潜力和未来在医疗实践中的应用前景。
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来源期刊
Pharmaceutics
Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.90
自引率
11.10%
发文量
2379
审稿时长
16.41 days
期刊介绍: Pharmaceutics (ISSN 1999-4923) is an open access journal which provides an advanced forum for the science and technology of pharmaceutics and biopharmaceutics. It publishes reviews, regular research papers, communications,  and short notes. Covered topics include pharmacokinetics, toxicokinetics, pharmacodynamics, pharmacogenetics and pharmacogenomics, and pharmaceutical formulation. Our aim is to encourage scientists to publish their experimental and theoretical details in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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