Different dietary compositions alter pubertal onset in Wistar rats.

IF 3.6 4区 医学 Q2 NEUROSCIENCES
Harsh Shah, Nehareeka Dan, Ankita Salunke, A V Ramachandran, Parth Pandya
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引用次数: 0

Abstract

Introduction: Puberty is a crucial developmental phase influenced by neuroendocrine signals involving various neuropeptides and hormones. The impact of various diet combinations on the initiation of puberty in female rats was studied, along with the crosstalk between hypothalamic neuropeptides that might influence it.

Methods: Weaned female Wistar rats were segregated into five groups and given different diets for a duration of 21 d. The diet included high-fat, high-carbohydrate, high-protein, cafeteria diet and standard chow. Throughout the 21-day period, body weight, vaginal opening and blood samples were recorded. Post sacrifice parameters like hormonal analysis, gene expression, protein expression and Immunolocalization were performed.

Results: The High Fat Diet (HFD), High carbohydrate Diet (HCD), and Cafeteria diet (CafD) groups exhibited early vaginal opening, increased body weight, elevated somatic indices, and Follicle-stimulating Hormone (FSH), Luteinizing Hormone (LH), and estradiol levels were elevated in regards to the control group. Gene expression analysis showed upregulation of Kiss1, Kiss1r, Pomc, Lep, Lepr, and Gnrh, while Npy, Agrp, and Mkrn3 were downregulated. Protein expression studies confirmed the increased levels of KISS1, KISS1R, LEPR, and POMC, particularly in the HFD and HCD groups. Histone acetylation analysis revealed higher global acetylation in the hypothalamus of HFD and HCD groups.

Discussion: This study highlights the significant role of dietary composition in modulating pubertal onset through the neuroendocrine pathways. These findings suggest that high-fat and high-carbohydrate diets expedite puberty by altering the expression of key hypothalamic neuropeptides and hormones. This underscores the importance of nutrition in reproductive development and its broad implications for adolescent health.

不同的饮食成分改变了Wistar大鼠的青春期。
青春期是一个重要的发育阶段,受神经内分泌信号的影响,包括各种神经肽和激素。研究了不同饮食组合对雌性大鼠青春期开始的影响,以及可能影响它的下丘脑神经肽之间的串扰。方法:将断奶雌性Wistar大鼠分为5组,分别饲喂高脂、高碳水化合物、高蛋白、自助饮食和标准饲料,为期21 d。在21天的时间里,记录体重、阴道口和血液样本。测定牺牲后的激素分析、基因表达、蛋白表达和免疫定位等参数。结果:高脂肪饮食组(HFD)、高碳水化合物饮食组(HCD)和自助饮食组(CafD)表现出阴道开放提前、体重增加、躯体指数升高,促卵泡激素(FSH)、黄体生成素(LH)和雌二醇水平较对照组升高。基因表达分析显示,Kiss1、Kiss1r、Pomc、Lep、Lepr、Gnrh表达上调,Npy、Agrp、Mkrn3表达下调。蛋白表达研究证实,KISS1、KISS1R、LEPR和POMC水平升高,特别是在HFD和HCD组。组蛋白乙酰化分析显示,HFD和HCD组下丘脑的整体乙酰化水平较高。讨论:本研究强调了饮食成分在通过神经内分泌途径调节青春期发病中的重要作用。这些发现表明,高脂肪和高碳水化合物饮食通过改变关键的下丘脑神经肽和激素的表达来加速青春期。这强调了营养在生殖发育中的重要性及其对青少年健康的广泛影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nutritional Neuroscience
Nutritional Neuroscience 医学-神经科学
CiteScore
8.50
自引率
2.80%
发文量
236
审稿时长
>12 weeks
期刊介绍: Nutritional Neuroscience is an international, interdisciplinary broad-based, online journal for reporting both basic and clinical research in the field of nutrition that relates to the central and peripheral nervous system. Studies may include the role of different components of normal diet (protein, carbohydrate, fat, moderate use of alcohol, etc.), dietary supplements (minerals, vitamins, hormones, herbs, etc.), and food additives (artificial flavours, colours, sweeteners, etc.) on neurochemistry, neurobiology, and behavioural biology of all vertebrate and invertebrate organisms. Ideally this journal will serve as a forum for neuroscientists, nutritionists, neurologists, psychiatrists, and those interested in preventive medicine.
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