FpFumB Is Required for Basic Biological Processes and Virulence in Fusarium proliferatum by Modulating DNA Repair Through Interaction with FpSae2.

IF 4.2 2区生物学 Q2 MICROBIOLOGY

Microorganisms Pub Date : 2025-06-19 DOI:10.3390/microorganisms13061433

Yizhou Gao, Haibo Li, Yong Liu, Yuqing Wang, Jingwen Xue, Yitong Wang, Zhihong Wu

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Abstract

Fumarase plays a pivotal role in the tricarboxylic acid cycle, but its functions in plant pathogenic fungi are not well understood. We identified two fumarase genes in Fusarium proliferatum and generated individual deletion mutants. Loss of FpFumB led to defects in growth, sporulation, stress tolerance, and virulence. Exogenous malate supplementation restored growth defects. Site-directed mutagenesis of residues G452 and A463 reduced FpFumB enzyme activity. Transcriptomic analysis identified significant changes in gene expression related to different metabolic pathways. Protein interaction assays showed that FpFumB interacts with the DNA repair protein FpSae2. Both ΔFpFumB and ΔFpSae2 mutants displayed altered sensitivity to DNA-damaging agents and reduced virulence, indicating that FpFumB modulates DNA repair and pathogenicity through its interaction with FpSae2. Together, these findings highlight FpFumB as a key regulator of basic biological processes, DNA damage repair, and virulence in Fusarium proliferatum.

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FpFumB通过与FpSae2相互作用调节DNA修复，是增殖镰刀菌基本生物过程和毒力所必需的。

延胡索酸酶在三羧酸循环中起关键作用，但其在植物病原真菌中的作用尚不清楚。我们在增殖镰刀菌中鉴定了两个延胡索酶基因，并产生了个体缺失突变体。FpFumB的缺失导致了生长、产孢、抗逆性和毒力的缺陷。外源苹果酸盐补充恢复生长缺陷。残基G452和A463的定点诱变降低了FpFumB酶的活性。转录组学分析发现了与不同代谢途径相关的基因表达的显著变化。蛋白相互作用实验表明，FpFumB与DNA修复蛋白FpSae2相互作用。ΔFpFumB和ΔFpSae2突变体都表现出对DNA损伤剂的敏感性改变和毒性降低，表明FpFumB通过与FpSae2的相互作用调节DNA修复和致病性。总之，这些发现强调FpFumB是增殖镰刀菌基本生物过程、DNA损伤修复和毒力的关键调节因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.