Antiviral Activity of Brazilian Propolis from Stingless Bees Against Rotavirus.

IF 4.2 2区生物学 Q2 MICROBIOLOGY

Microorganisms Pub Date : 2025-06-19 DOI:10.3390/microorganisms13061424

Guadalupe González-Ochoa, Ana Paola Balderrama-Carmona, Jesús Antonio Erro-Carvajal, José Guadalupe Soñanez-Organis, Luis Alberto Zamora-Álvarez, Marcelo Andrés Umsza Guez

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引用次数: 0

Abstract

Group A rotavirus (RV) causes gastrointestinal disease in infants worldwide, and there is currently no specific treatment to eliminate the virus. Due to its chemical properties, propolis is a promising compound for improving gastrointestinal infections. This study aimed to evaluate the action of stingless bee propolis against RV. The method involved determining the concentrations of the extracts that do not exhibit cytotoxicity in colon adenocarcinoma cells using the MTT assay and measuring the reduction in infectivity through a focus forming assay. The results showed that stingless bee propolis was non-cytotoxic up to 200 µg/mL. The reduction in RV infectivity exceeded 99% when using propolis from Plebeia droryana and Melipona quadrifasciata. Brazilian stingless bee propolis, whose active components are known for their activity against various viruses, was experimentally tested and demonstrated effective antiviral activity against RV, supporting its potential application as an antiviral agent.

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巴西无刺蜂蜂胶对轮状病毒的抗病毒活性研究。

A组轮状病毒（RV）在世界范围内引起婴儿胃肠道疾病，目前没有专门的治疗方法来消除这种病毒。由于其化学性质，蜂胶是一种有希望改善胃肠道感染的化合物。本研究旨在评价无刺蜂胶对RV的防治作用。该方法包括使用MTT试验确定在结肠腺癌细胞中不表现出细胞毒性的提取物的浓度，并通过焦点形成试验测量传染性的降低。结果表明，无刺蜂胶在200µg/mL浓度下无细胞毒性。用白蛉蜂胶和四筋蜂蜂胶对RV的传染性降低超过99%。巴西无刺蜂蜂胶的有效成分以其对多种病毒的活性而闻名，实验测试表明，它对RV具有有效的抗病毒活性，支持其作为抗病毒剂的潜在应用。

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来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.