Expression of cellulase in Candida glycerogenes and strengthen the expression level for application in residue-containing fermentation to enhance glycerol production.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2025-06-27 DOI:10.1007/s10529-025-03602-7

Yuxin Gao, Dongqi Jiang, Mengying Wang, Xueqing Du, Hong Zong, Bin Zhuge

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Abstract

Objectives: Expressing cellulase systems in C. glycerinogenes with extracellular secretion ability, and using fermentation with residue, enabled the recombinant strain to degrade lignocellulosic waste for efficient glycerol production, offering new option for agricultural waste transformation.

Results: Candida glycerinogenes is employed as the host strain, various cellulases were screened. Signal peptide mining and screening, and semi-rational design strategy was adopted in the host strain. The recombinant strain Cg4 had a total cellulase activity of 13.6 U/mL. Cg4 was applied to 110 g/L sugarcane bagasse hydrolysate with 40 g/L of pretreated bagasse as the substrate. The glycerol yield reached 50 g/L, with a 43.3% bagasse utilization rate, the amount of cellulase used in the degradation of lignocellulose was reduced by 12.1%.

Conclusion: Opened up a route for efficient degradation of agricultural waste of lignocellulose by C. glycerinogenes for glycerol production, the emission of lignocellulose waste had been reduced, and decreasing the need for cellulases in hydrolysis.

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纤维素酶在产甘油假丝酵母中的表达及强化表达水平，用于含渣发酵提高甘油产量。

目的：在C. glycerinogenes中表达具有胞外分泌能力的纤维素酶体系，并利用残渣发酵，使重组菌株能够降解木质纤维素废弃物，高效生产甘油，为农业废弃物转化提供新的选择。结果：以产甘油假丝酵母为宿主菌株，筛选出多种纤维素酶。在宿主菌株中采用信号肽挖掘筛选和半理性设计策略。重组菌株Cg4的总纤维素酶活性为13.6 U/mL。以40 g/L预处理甘蔗渣为底物，将Cg4添加到110 g/L蔗渣水解液中。甘油产率达到50 g/L，蔗渣利用率为43.3%，降解木质纤维素的纤维素酶用量减少12.1%。结论：为产甘油原菌高效降解农业废弃物木质纤维素开辟了一条途径，减少了木质纤维素废弃物的排放，减少了对纤维素酶的水解需求。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.