Unraveling viral protein-host membrane interaction for dengue and Zika.

IF 3.2 3区 生物学 Q2 BIOPHYSICS
Natalia Philipp, Guadalupe S Costa Navarro, Luana de Borba, Andrea V Gamarnik, Laura C Estrada
{"title":"Unraveling viral protein-host membrane interaction for dengue and Zika.","authors":"Natalia Philipp, Guadalupe S Costa Navarro, Luana de Borba, Andrea V Gamarnik, Laura C Estrada","doi":"10.1016/j.bpj.2025.06.032","DOIUrl":null,"url":null,"abstract":"<p><p>Mosquito-borne flaviviruses, including dengue virus (DENV) and Zika virus (ZIKV), constitute a significant and escalating public health threat. Elucidating the mechanisms by which these flaviviruses subvert cellular processes through viral protein-host cell interactions provides critical insights into their replication and pathogenicity. Here, we present an analysis based on anisotropy calculation across pixels in raster images to investigate differential protein interactions during nucleocytoplasmic shuttling. This methodology highlights regions of high and low anisotropy, suggesting differential protein-membrane interactions. Employing numerical simulations and confocal raster images, we use this method to investigate capsid (C) protein-host cell membrane interactions for dengue virus serotype 2 (DENV2) and ZIKV-transfected cells during C-protein nucleocytoplasmic shuttling. By 2D pair correlation analysis on infected cells, we demonstrated that the DENV2 C-protein exhibits a pronounced interaction with the host cell's nuclear membrane. Furthermore, we identified a differential localization of C-protein for these viruses in the host cell. These findings highlight the significance of comparative viral protein-host interaction mapping in deciphering pathogenic mechanisms, particularly when combined with in vivo models. Given that dengue and Zika are two of the most significant human viral infections transmitted by arthropods, a thorough understanding of viral protein dynamics is imperative for developing novel antiviral strategies.</p>","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysical journal","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.bpj.2025.06.032","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0

Abstract

Mosquito-borne flaviviruses, including dengue virus (DENV) and Zika virus (ZIKV), constitute a significant and escalating public health threat. Elucidating the mechanisms by which these flaviviruses subvert cellular processes through viral protein-host cell interactions provides critical insights into their replication and pathogenicity. Here, we present an analysis based on anisotropy calculation across pixels in raster images to investigate differential protein interactions during nucleocytoplasmic shuttling. This methodology highlights regions of high and low anisotropy, suggesting differential protein-membrane interactions. Employing numerical simulations and confocal raster images, we use this method to investigate capsid (C) protein-host cell membrane interactions for dengue virus serotype 2 (DENV2) and ZIKV-transfected cells during C-protein nucleocytoplasmic shuttling. By 2D pair correlation analysis on infected cells, we demonstrated that the DENV2 C-protein exhibits a pronounced interaction with the host cell's nuclear membrane. Furthermore, we identified a differential localization of C-protein for these viruses in the host cell. These findings highlight the significance of comparative viral protein-host interaction mapping in deciphering pathogenic mechanisms, particularly when combined with in vivo models. Given that dengue and Zika are two of the most significant human viral infections transmitted by arthropods, a thorough understanding of viral protein dynamics is imperative for developing novel antiviral strategies.

揭示登革热和寨卡病毒蛋白-宿主膜相互作用。
蚊媒黄病毒,包括登革热病毒(DENV)和寨卡病毒(ZIKV),构成重大和不断升级的公共卫生威胁。阐明这些黄病毒通过病毒蛋白-宿主细胞相互作用破坏细胞过程的机制,为了解它们的复制和致病性提供了重要的见解。在这里,我们提出了一个基于光栅图像中像素各向异性计算的分析,以研究核细胞质穿梭过程中的差异蛋白质相互作用。这种方法突出了高各向异性和低各向异性的区域,表明不同的蛋白质-膜相互作用。利用数值模拟和共聚焦光栅图像,研究了登革热病毒血清2型(DENV2)和zikv转染细胞在C蛋白核胞质穿梭过程中衣壳(C)蛋白与宿主细胞膜的相互作用。通过对感染细胞的二维对相关分析,我们证明了DENV2 c蛋白与宿主细胞的核膜表现出明显的相互作用。此外,我们确定了这些病毒在宿主细胞中c蛋白的差异定位。这些发现强调了比较病毒蛋白-宿主相互作用图谱在破译致病机制中的重要性,特别是当与体内模型相结合时。鉴于登革热和寨卡是节肢动物传播的两种最重要的人类病毒感染,彻底了解病毒蛋白动力学对于开发新的抗病毒策略至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biophysical journal
Biophysical journal 生物-生物物理
CiteScore
6.10
自引率
5.90%
发文量
3090
审稿时长
2 months
期刊介绍: BJ publishes original articles, letters, and perspectives on important problems in modern biophysics. The papers should be written so as to be of interest to a broad community of biophysicists. BJ welcomes experimental studies that employ quantitative physical approaches for the study of biological systems, including or spanning scales from molecule to whole organism. Experimental studies of a purely descriptive or phenomenological nature, with no theoretical or mechanistic underpinning, are not appropriate for publication in BJ. Theoretical studies should offer new insights into the understanding ofexperimental results or suggest new experimentally testable hypotheses. Articles reporting significant methodological or technological advances, which have potential to open new areas of biophysical investigation, are also suitable for publication in BJ. Papers describing improvements in accuracy or speed of existing methods or extra detail within methods described previously are not suitable for BJ.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信