Comparative analysis of the effect of hybrid vaterite microparticles with various polysaccharides on neutrophils activity.

Q3 Biochemistry, Genetics and Molecular Biology

Biomeditsinskaya khimiya Pub Date : 2025-06-01 DOI:10.18097/PBMCR1561

D V Grigorieva, E V Mikhalchik, D V Mosievich, P I Mishin, N G Balabushevich, O M Panasenko, A V Sokolov, I V Gorudko

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引用次数: 0

Abstract

Carriers based on natural biominerals attract much attention in the context of the development of new drug delivery systems. In this study, the effects of native (CC) and hybrid vaterite microparticles with the inclusion of dextran sulfate (CCDS), chondroitin sulfate (CCCS), heparin (CCHE), fucoidan (CCFU), and pectin (CCPE) have been investigated on the viability and functional activity of neutrophils. Among the tested preparations, only CCFU exhibited a slight cytotoxic effect. Native CC stimulated actin cytoskeleton rearrangements and cell production of reactive oxygen species (ROS), which decreased in the presence of diphenyleneiodonium chloride (DPI), an inhibitor of NADPH oxidase assembly. The CC-induced NADPH oxidase activation was reduced in the presence of inhibitors of non-receptor tyrosine kinases of the Src family, phosphatidylinositol 3-kinase (PI3K), and phospholipase C (PLC). Similar to native CC, hybrid vaterite microparticles also initiated ROS production by neutrophils. After addition of CC and hybrid vaterite microparticles (except CCDS), an increase in the number of neutrophils characterized by higher values of the side scattering value was detected thus indicating a change in the morphological characteristics of the cells. Given the ability of hybrid vaterite microparticles with polysaccharides to activate neutrophil NADPH oxidase, they could be promising systems for the delivery of antibacterial and antiviral drugs.

查看原文本刊更多论文

不同多糖杂化水晶石微粒对中性粒细胞活性影响的比较分析。

基于天然生物矿物质的载体在新型给药系统的开发中备受关注。本研究研究了含有硫酸葡聚糖（CCDS）、硫酸软骨素（CCCS）、肝素（CCHE）、岩藻聚糖（CCFU）和果胶（CCPE）的天然（CC）和杂交水晶石微粒对中性粒细胞活力和功能活性的影响。在被试制剂中，只有CCFU表现出轻微的细胞毒性作用。天然CC刺激肌动蛋白细胞骨架重排和细胞活性氧（ROS）的产生，在NADPH氧化酶组装抑制剂二苯二氯铵（DPI）存在下，活性氧（ROS）的产生减少。在Src家族非受体酪氨酸激酶、磷脂酰肌醇3-激酶（PI3K）和磷脂酶C （PLC）抑制剂的存在下，cc诱导的NADPH氧化酶活化降低。与原生CC类似，杂交鼠体微粒也通过中性粒细胞启动ROS的产生。在加入CC和杂交vaterite微粒子（CCDS除外）后，检测到以较高侧散射值为特征的中性粒细胞数量增加，从而表明细胞形态特征发生了变化。考虑到含有多糖的杂化水蛭微粒能够激活中性粒细胞NADPH氧化酶，它们可能是有希望的抗菌和抗病毒药物递送系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).