Resolving relationships in the radiation of Australia's largest pea clade (Fabaceae tribe Mirbelieae) with target-capture sequencing.

IF 3.6 2区生物学 Q1 PLANT SCIENCES

Annals of botany Pub Date : 2025-06-17 DOI:10.1093/aob/mcaf128

James A R Clugston, Russell L Barrett, Daniel J Murphy, Matthew A M Renner, Peter H Weston, Lyn G Cook, Peter C Jobson, Brendan J Lepschi, Michael D Crisp

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引用次数: 0

Abstract

Background and aims: Tribe Mirbelieae (Fabaceae) represents one of the great species radiations in Australian flora and the largest in the pea-flowered legumes. Traditional amplicon sequencing has failed to resolve relationships within this species-rich and morphologically diverse tribe.

Methods: Target capture sequencing was used to reconstruct relationships within the core Mirbelioid legumes which represent a previously hypothesised rapid radiation that dates to the Oligocene and early Miocene epochs.

Key results: We recovered strongly supported deep nodes resolving relationships between all recognised genera and four novel clades based on 289 low-copy nuclear markers derived from the Angiosperms353 universal probe set. The taxonomically challenging genus Pultenaea was demonstrated to be polyphyletic. Minor changes are required in Aotus, Callistachys, Mirbelia, Oxylobium, Phyllota and Urodon.

Conclusions: Phylogenomic data has robustly resolved relationships in a large legume clade where relationships long-feared irresolvable. This resolution enables the recognition of monophyletic genera within the tribe with only minimal taxonomic rearrangements. Critically, a new circumscription of Pultenaea supported by both phylogenomic and morphological data has now been achieved.

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用目标捕获测序解决澳大利亚最大的豌豆分支（豆科部落Mirbelieae）辐射中的关系。

背景与目的：豆科（Tribe Mirbelieae）是澳大利亚植物区系中最大的物种辐射之一，也是豆科中最大的物种。传统的扩增子测序已经无法解决这个物种丰富和形态多样的部落之间的关系。方法：目标捕获测序用于重建核心Mirbelioid豆科植物之间的关系，这些豆科植物代表了先前假设的可追溯到渐新世和中新世早期的快速辐射。关键结果：基于来自Angiosperms353通用探针集的289个低拷贝核标记，我们恢复了强支持的深节点，解决了所有识别属和四个新枝之间的关系。在分类学上具有挑战性的属Pultenaea被证明是多系的。Aotus、Callistachys、Mirbelia、Oxylobium、Phyllota和Urodon需要进行微小的改变。结论：系统基因组学数据已经强有力地解决了一个大型豆科进化枝的关系，而长期以来人们担心这种关系无法解决。这一决议使识别单系属内的部落只有最小的分类重排。重要的是，系统基因组学和形态学数据支持的Pultenaea的新定义现在已经实现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of botany 生物-植物科学

CiteScore

7.90

自引率

4.80%

发文量

138

审稿时长

3 months

期刊介绍： Annals of Botany is an international plant science journal publishing novel and rigorous research in all areas of plant science. It is published monthly in both electronic and printed forms with at least two extra issues each year that focus on a particular theme in plant biology. The Journal is managed by the Annals of Botany Company, a not-for-profit educational charity established to promote plant science worldwide. The Journal publishes original research papers, invited and submitted review articles, ''Research in Context'' expanding on original work, ''Botanical Briefings'' as short overviews of important topics, and ''Viewpoints'' giving opinions. All papers in each issue are summarized briefly in Content Snapshots , there are topical news items in the Plant Cuttings section and Book Reviews . A rigorous review process ensures that readers are exposed to genuine and novel advances across a wide spectrum of botanical knowledge. All papers aim to advance knowledge and make a difference to our understanding of plant science.