Genetic tools for imaging microcirculation via plasma labeling.

IF 1.2 4区 医学 Q3 ANATOMY & MORPHOLOGY
Xiaowen Wang, Marta Vittani, Ashley Bomin Lee, Philip Gade Knak, Hajime Hirase
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引用次数: 0

Abstract

Cerebral microcirculation is a critical infrastructure for brain function, delivering energy substrates and clearing metabolic byproducts. Disruptions in vascular dynamics contribute to neurodegenerative diseases, stroke, and cognitive impairments. Traditional blood labeling methods for fluorescence imaging, such as fluorescent dextran injection, have advanced our understanding of microcirculation but are limited for long-term imaging. In this mini review, we introduce two recently developed molecular genetic techniques, achieved by recombinant adeno-associated virus (AAV)-mediated plasma label expression or genomic knock-in that enable stable, long-term microcirculation imaging. These AAV-mediated methods require only a single systemic injection, facilitating longitudinal imaging of microcirculation in mouse models of disease. We discuss the fundamental design concepts of these approaches and explore their potential applications in systems biology.

通过血浆标记成像微循环的遗传工具。
脑微循环是脑功能的关键基础设施,传递能量底物和清除代谢副产物。血管动力学紊乱可导致神经退行性疾病、中风和认知障碍。用于荧光成像的传统血液标记方法,如荧光葡聚糖注射,促进了我们对微循环的理解,但对长期成像有限制。在这篇综述中,我们介绍了两种最新开发的分子遗传技术,通过重组腺相关病毒(AAV)介导的血浆标记表达或基因组敲入实现了稳定、长期的微循环成像。这些aav介导的方法只需要一次全身注射,便于在小鼠疾病模型中进行微循环纵向成像。我们讨论了这些方法的基本设计概念,并探讨了它们在系统生物学中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Anatomical Science International
Anatomical Science International 医学-解剖学与形态学
CiteScore
2.80
自引率
8.30%
发文量
50
审稿时长
>12 weeks
期刊介绍: The official English journal of the Japanese Association of Anatomists, Anatomical Science International (formerly titled Kaibogaku Zasshi) publishes original research articles dealing with morphological sciences. Coverage in the journal includes molecular, cellular, histological and gross anatomical studies on humans and on normal and experimental animals, as well as functional morphological, biochemical, physiological and behavioral studies if they include morphological analysis.
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