Atmospheric and room temperature plasma mutagenesis of Graesiella emersonii for enhanced protein production using methanol as novel carbon source

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-06-24 DOI:10.1016/j.biortech.2025.132880

Jixu Luo , Guanqin Huang , Mingjia Zheng , Changqing Ou , Qingyue Chen , Yufeng Ling , Linlin Cai , Yang Yang , Lingcheng Liu , Fei Huang , Zhangli Hu , Yihong Zheng

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Abstract

Methanol presents a promising alternative carbon source for microalgal cultivation, yet its effective utilization remains challenging. This study generated methanol-tolerant Graesiella emersonii strain through Atmospheric and Room Temperature Plasma mutagenesis. The selected mutant exhibited 60% higher growth rate than wild-type in methanol-supplemented medium. Comparative analysis revealed enhanced photosynthetic efficiency, nitrogen assimilation, and carbon fixation in the mutant strain, enabling 27.1% greater methanol consumption. Biochemical profiling demonstrated simultaneous enhancement of carbohydrate (40%) and protein (40%) accumulation with improved essential amino acid composition. Fatty acid analysis showed increased membrane unsaturation as an adaptive response to methanol stress. Additionally, the mutant strain accumulated higher levels of valuable carotenoids, particularly astaxanthin. These findings establish a foundation for methanol-based microalgal biorefineries producing high-quality protein alongside valuable pigments, offering both economic and environmental benefits through industrial methanol utilization.

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以甲醇为新型碳源的大气和室温等离子体诱变爱默生氏格雷氏菌提高蛋白质产量

甲醇是一种很有前途的微藻碳源，但其有效利用仍具有挑战性。本研究通过常压等离子体诱变和室温等离子体诱变制备了耐甲醇的爱默生氏格雷氏菌菌株。所选突变体在添加甲醇培养基中的生长速度比野生型高出60%。对比分析显示，突变菌株的光合效率、氮同化和固碳能力增强，甲醇消耗量增加34%。生化分析表明，碳水化合物（40%）和蛋白质（40%）的积累同时增加，必需氨基酸组成也有所改善。脂肪酸分析表明，膜不饱和增加是对甲醇胁迫的适应性反应。此外，突变菌株积累了更高水平的有价值的类胡萝卜素，特别是虾青素。这些发现为以甲醇为基础的微藻生物精炼厂生产高质量的蛋白质和有价值的色素奠定了基础，通过工业甲醇利用提供了经济和环境效益。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.