{"title":"Ultrasmall Rh-Mo Nanoparticles with Prominent Peroxidase Activity for Colorimetric Sensing of L-Cysteine.","authors":"Yayao Wang, Xinyan Hu, Gongxin Zhang, Lida Tang, Xiaolan Chen","doi":"10.1002/asia.202500613","DOIUrl":null,"url":null,"abstract":"<p><p>Nanozymes with peroxidase-mimicking properties have emerged as attractive candidates for diverse biomedical applications. In this study, we first prepared ultrasmall rhodium-molybdenum nanoparticles (Rh-Mo NPs) via a straightforward hydrothermal method, which exhibited remarkable peroxidase (POD)-like catalytic activity. These nanoparticles effectively catalyzed the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ to generate blue oxidized TMB (ox-TMB), demonstrating superior catalytic performance and substrate specificity. Leveraging this exceptional enzymatic activity, we established a novel colorimetric biosensing platform for sensitive determination of L-cysteine (L-Cys). Experimental data confirmed dual linear absorbance-concentration correlations for L-Cys (0.01-10.0 µM, limit of detection (LOD) = 3.3 nM; 10.0-40.0 µM, LOD = 26.5 nM), exhibiting a segmented dose-response pattern over the 0.01-40.0 µM detection range. The sensing system has three significant advantages: (1) intuitive operation requiring minimal technical expertise, (2) accelerated analysis completion within 5 min, and (3) exceptional sensitivity with low LOD. Notably, the Rh-Mo NPs-based sensing platform could successfully detect endogenous L-Cys in complex biological matrices, including serum and milk samples, confirming their practical applicability in real-sample analysis.</p>","PeriodicalId":145,"journal":{"name":"Chemistry - An Asian Journal","volume":" ","pages":"e00613"},"PeriodicalIF":3.5000,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry - An Asian Journal","FirstCategoryId":"1","ListUrlMain":"https://doi.org/10.1002/asia.202500613","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Nanozymes with peroxidase-mimicking properties have emerged as attractive candidates for diverse biomedical applications. In this study, we first prepared ultrasmall rhodium-molybdenum nanoparticles (Rh-Mo NPs) via a straightforward hydrothermal method, which exhibited remarkable peroxidase (POD)-like catalytic activity. These nanoparticles effectively catalyzed the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ to generate blue oxidized TMB (ox-TMB), demonstrating superior catalytic performance and substrate specificity. Leveraging this exceptional enzymatic activity, we established a novel colorimetric biosensing platform for sensitive determination of L-cysteine (L-Cys). Experimental data confirmed dual linear absorbance-concentration correlations for L-Cys (0.01-10.0 µM, limit of detection (LOD) = 3.3 nM; 10.0-40.0 µM, LOD = 26.5 nM), exhibiting a segmented dose-response pattern over the 0.01-40.0 µM detection range. The sensing system has three significant advantages: (1) intuitive operation requiring minimal technical expertise, (2) accelerated analysis completion within 5 min, and (3) exceptional sensitivity with low LOD. Notably, the Rh-Mo NPs-based sensing platform could successfully detect endogenous L-Cys in complex biological matrices, including serum and milk samples, confirming their practical applicability in real-sample analysis.
期刊介绍:
Chemistry—An Asian Journal is an international high-impact journal for chemistry in its broadest sense. The journal covers all aspects of chemistry from biochemistry through organic and inorganic chemistry to physical chemistry, including interdisciplinary topics.
Chemistry—An Asian Journal publishes Full Papers, Communications, and Focus Reviews.
A professional editorial team headed by Dr. Theresa Kueckmann and an Editorial Board (headed by Professor Susumu Kitagawa) ensure the highest quality of the peer-review process, the contents and the production of the journal.
Chemistry—An Asian Journal is published on behalf of the Asian Chemical Editorial Society (ACES), an association of numerous Asian chemical societies, and supported by the Gesellschaft Deutscher Chemiker (GDCh, German Chemical Society), ChemPubSoc Europe, and the Federation of Asian Chemical Societies (FACS).