Ultrasmall Rh-Mo Nanoparticles with Prominent Peroxidase Activity for Colorimetric Sensing of L-Cysteine.

IF 3.5 3区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Yayao Wang, Xinyan Hu, Gongxin Zhang, Lida Tang, Xiaolan Chen
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引用次数: 0

Abstract

Nanozymes with peroxidase-mimicking properties have emerged as attractive candidates for diverse biomedical applications. In this study, we first prepared ultrasmall rhodium-molybdenum nanoparticles (Rh-Mo NPs) via a straightforward hydrothermal method, which exhibited remarkable peroxidase (POD)-like catalytic activity. These nanoparticles effectively catalyzed the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ to generate blue oxidized TMB (ox-TMB), demonstrating superior catalytic performance and substrate specificity. Leveraging this exceptional enzymatic activity, we established a novel colorimetric biosensing platform for sensitive determination of L-cysteine (L-Cys). Experimental data confirmed dual linear absorbance-concentration correlations for L-Cys (0.01-10.0 µM, limit of detection (LOD) = 3.3 nM; 10.0-40.0 µM, LOD = 26.5 nM), exhibiting a segmented dose-response pattern over the 0.01-40.0 µM detection range. The sensing system has three significant advantages: (1) intuitive operation requiring minimal technical expertise, (2) accelerated analysis completion within 5 min, and (3) exceptional sensitivity with low LOD. Notably, the Rh-Mo NPs-based sensing platform could successfully detect endogenous L-Cys in complex biological matrices, including serum and milk samples, confirming their practical applicability in real-sample analysis.

具有过氧化物酶活性的超小Rh-Mo纳米颗粒用于l -半胱氨酸的比色检测。
具有过氧化物酶模拟特性的纳米酶已成为各种生物医学应用的有吸引力的候选者。在这项研究中,我们首先通过简单的水热法制备了超小的铑钼纳米颗粒(Rh-Mo NPs),该纳米颗粒具有显著的过氧化物酶(POD)样催化活性。这些纳米颗粒在h2o2存在下有效催化无色的3,3',5,5'-四甲基联苯胺(TMB)氧化生成蓝色氧化TMB (x-TMB),表现出优异的催化性能和底物特异性。利用这种特殊的酶活性,我们建立了一种新的比色生物传感平台,用于l -半胱氨酸(L-Cys)的灵敏测定。L-Cys的吸光度-浓度呈双线性相关关系(0.01 ~ 10.0µM),检出限(LOD) = 3.3 nM;10.0 ~ 40.0µM, LOD = 26.5 nM),在0.01 ~ 40.0µM检测范围内呈现分段剂量响应模式。该传感系统具有三个显著优势:(1)操作直观,仅需最少的专业技术知识;(2)在5分钟内加速分析完成;(3)低LOD的卓越灵敏度。值得注意的是,基于Rh-Mo nps的传感平台可以成功检测血清和牛奶样品等复杂生物基质中的内源性L-Cys,证实了其在实际样品分析中的实用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Chemistry - An Asian Journal
Chemistry - An Asian Journal 化学-化学综合
CiteScore
7.00
自引率
2.40%
发文量
535
审稿时长
1.3 months
期刊介绍: Chemistry—An Asian Journal is an international high-impact journal for chemistry in its broadest sense. The journal covers all aspects of chemistry from biochemistry through organic and inorganic chemistry to physical chemistry, including interdisciplinary topics. Chemistry—An Asian Journal publishes Full Papers, Communications, and Focus Reviews. A professional editorial team headed by Dr. Theresa Kueckmann and an Editorial Board (headed by Professor Susumu Kitagawa) ensure the highest quality of the peer-review process, the contents and the production of the journal. Chemistry—An Asian Journal is published on behalf of the Asian Chemical Editorial Society (ACES), an association of numerous Asian chemical societies, and supported by the Gesellschaft Deutscher Chemiker (GDCh, German Chemical Society), ChemPubSoc Europe, and the Federation of Asian Chemical Societies (FACS).
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