New Click on the Block: Photocaged Hydroxylamine, an Addition to the Light-Activatable Bioorthogonal Toolbox.

Abstract

Krisztina Németh,†,[a] Flóra Horváth,†,[a] Ágnes Szatmári,[a] Evelin Albitz,[a] Péter Kele*[a] and Gergely B. Cserép*[a] A new photoactivatable bioorthogonal function was added to the photoactivatable bioorthogonal toolbox by transiently disabling the bioorthogonal reactivity of the hydroxylamine moiety with a photolabile protecting group. The photocaged bioorthogonal function was further equipped with a HaloTag substrate to enable its use in live-cell labeling schemes. Light-controlled recovery of reactivity was demonstrated by bioorthogonal fluorescent labeling of intracellular proteins in live cells.