Lipopolysaccharide nanoparticles, a biomimetic platform to study bacterial surface.

IF 3.2 3区生物学 Q2 BIOPHYSICS

Biophysical journal Pub Date : 2025-06-27 DOI:10.1016/j.bpj.2025.06.036

Massilia Abbas, Samantha Micciulla, Jean-Marie Teulon, Meriem Maalej, Macha Trembley, Roberta Marchetti, Antonio Molinaro, Michel Thépaut, Franck Fieschi, Jean-Luc Pellequer, Cédric Laguri

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引用次数: 0

Abstract

Lipopolysaccharides (LPS) are essential components of the outer membranes of Gram-negative bacteria, playing a crucial role in antimicrobial resistance, virulence, and the host’s immune response. Self-assembled particles displaying LPS are essential for biophysical studies addressing the behaviour of bacterial surfaces under specific biomimetic conditions. Styrene-maleic acid (SMA) copolymers were employed to form LPS nanoparticles, either from extracted LPS or directly from purified outer membranes. These nanoparticles, derived from pathogenic O157:H7 or laboratory E. coli strains, are well-defined in size and yield high-resolution nuclear magnetic resonance (NMR) spectra. They have been successfully used to investigate molecular recognition by a human C-type Lectin Receptor (CLR) of the immune system and interaction with polymyxin antibiotics using various biophysical methods. This study highlights the significance of LPS nanoparticles as bacterial surface mimetics and opens promising avenues for further research into LPS structure and interactions. The ability to generate well-defined LPS nanoparticles offers a powerful tool for studying the molecular mechanisms underlying bacterial pathogenesis and immune response.

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脂多糖纳米颗粒，研究细菌表面的仿生平台。

脂多糖（LPS）是革兰氏阴性菌外膜的重要组成部分，在抗微生物药物耐药性、毒力和宿主免疫反应中起着至关重要的作用。显示LPS的自组装颗粒对于解决特定仿生条件下细菌表面行为的生物物理研究至关重要。苯乙烯-马来酸（SMA）共聚物通过提取LPS或直接从纯化的外膜制备LPS纳米颗粒。这些纳米颗粒来自致病性O157:H7或实验室大肠杆菌菌株，尺寸明确，并产生高分辨率核磁共振（NMR）光谱。他们已经成功地利用各种生物物理方法研究了人类c型凝集素受体（CLR）对免疫系统的分子识别以及与多粘菌素抗生素的相互作用。这项研究强调了LPS纳米颗粒作为细菌表面模拟物的重要性，并为进一步研究LPS的结构和相互作用开辟了有希望的途径。生成定义良好的LPS纳米颗粒的能力为研究细菌发病机制和免疫反应的分子机制提供了有力的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biophysical journal 生物-生物物理

CiteScore

6.10

自引率

5.90%

发文量

3090

审稿时长

2 months

期刊介绍： BJ publishes original articles, letters, and perspectives on important problems in modern biophysics. The papers should be written so as to be of interest to a broad community of biophysicists. BJ welcomes experimental studies that employ quantitative physical approaches for the study of biological systems, including or spanning scales from molecule to whole organism. Experimental studies of a purely descriptive or phenomenological nature, with no theoretical or mechanistic underpinning, are not appropriate for publication in BJ. Theoretical studies should offer new insights into the understanding ofexperimental results or suggest new experimentally testable hypotheses. Articles reporting significant methodological or technological advances, which have potential to open new areas of biophysical investigation, are also suitable for publication in BJ. Papers describing improvements in accuracy or speed of existing methods or extra detail within methods described previously are not suitable for BJ.