Activation of Visual Rhodopsin Probed by Single-Shot Transient IR Spectroscopy

IF 3.2 3区生物学 Q2 BIOPHYSICS

Biophysical journal Pub Date : 2025-06-28 DOI:10.1016/j.bpj.2025.06.030

Luiz Schubert, Franz Bartl, Joachim Heberle

引用次数: 0

Abstract

In order to understand the structure-function relationships of proteins, it is important to study their dynamics under physiological conditions. The advent of X-ray free electron lasers has made it possible to obtain the three-dimensional structures of proteins and their reaction intermediates at room temperature. However, these experiments are very demanding and require extensive planning. Here, we demonstrate that time-resolved infrared difference spectroscopy using quantum cascade lasers is a powerful tool for studying the dynamics of protein conformational changes. This method complements structural biology experiments and aids in data interpretation. We demonstrate the feasibility of studying the irreversible photoreaction of the G-protein-coupled receptor rhodopsin using single shots.

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单次瞬态红外光谱探测视紫红质的激活

为了了解蛋白质的结构-功能关系，研究蛋白质在生理条件下的动态是很重要的。x射线自由电子激光器的出现使得在室温下获得蛋白质及其反应中间体的三维结构成为可能。然而，这些实验要求很高，需要广泛的计划。在这里，我们证明了使用量子级联激光器的时间分辨红外差分光谱是研究蛋白质构象变化动力学的有力工具。这种方法补充了结构生物学实验，并有助于数据解释。我们证明了用单镜头研究g蛋白偶联受体视紫红质不可逆光反应的可行性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biophysical journal 生物-生物物理

CiteScore

6.10

自引率

5.90%

发文量

3090

审稿时长

2 months

期刊介绍： BJ publishes original articles, letters, and perspectives on important problems in modern biophysics. The papers should be written so as to be of interest to a broad community of biophysicists. BJ welcomes experimental studies that employ quantitative physical approaches for the study of biological systems, including or spanning scales from molecule to whole organism. Experimental studies of a purely descriptive or phenomenological nature, with no theoretical or mechanistic underpinning, are not appropriate for publication in BJ. Theoretical studies should offer new insights into the understanding ofexperimental results or suggest new experimentally testable hypotheses. Articles reporting significant methodological or technological advances, which have potential to open new areas of biophysical investigation, are also suitable for publication in BJ. Papers describing improvements in accuracy or speed of existing methods or extra detail within methods described previously are not suitable for BJ.