Exploiting glycan arrays and biosensors to diagnose and understand diseases

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2025-06-28 DOI:10.1016/j.biotechadv.2025.108635

Ae Sol Lee , Hye Ryoung Heo , Young Hoon Song , Chang Sup Kim , Jeong Hyun Seo , Hyung Joon Cha

{"title":"Exploiting glycan arrays and biosensors to diagnose and understand diseases","authors":"Ae Sol Lee , Hye Ryoung Heo , Young Hoon Song , Chang Sup Kim , Jeong Hyun Seo , Hyung Joon Cha","doi":"10.1016/j.biotechadv.2025.108635","DOIUrl":null,"url":null,"abstract":"<div><div>Aberrant glycosylation patterns, often observed in disease states compared to those in normal states, are involved in disease-related processes such as cancer cell development and metastasis. Analyzing glycan-associated interactions could provide promising avenues for disease detection and therapeutic development. Over the past two decades, glycan arrays and biosensors have become powerful analytical tools for characterizing glycan-associated interactions, screening functional glycans, and detecting glycan-related diseases. This review aims to describe glycan source preparation and immobilization methods used to construct glycan arrays and biosensors. We summarize methods for obtaining glycans from natural sources and for chemical and enzymatic synthesis. In particular, we cover a method of immobilizing DNA, proteins, and lipids that mimic cell-surface glycoconjugates. Finally, we discuss the biomedical applications of glycan arrays and biosensors, including identification of cancer-specific glycan biomarkers, evaluation of glycan-induced antibody responses in cancer, cancer detection, analysis of glycan-binding specificities of pathogens and their toxins, pathogen detection, and drug discovery.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"83 ","pages":"Article 108635"},"PeriodicalIF":12.5000,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology advances","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0734975025001211","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Aberrant glycosylation patterns, often observed in disease states compared to those in normal states, are involved in disease-related processes such as cancer cell development and metastasis. Analyzing glycan-associated interactions could provide promising avenues for disease detection and therapeutic development. Over the past two decades, glycan arrays and biosensors have become powerful analytical tools for characterizing glycan-associated interactions, screening functional glycans, and detecting glycan-related diseases. This review aims to describe glycan source preparation and immobilization methods used to construct glycan arrays and biosensors. We summarize methods for obtaining glycans from natural sources and for chemical and enzymatic synthesis. In particular, we cover a method of immobilizing DNA, proteins, and lipids that mimic cell-surface glycoconjugates. Finally, we discuss the biomedical applications of glycan arrays and biosensors, including identification of cancer-specific glycan biomarkers, evaluation of glycan-induced antibody responses in cancer, cancer detection, analysis of glycan-binding specificities of pathogens and their toxins, pathogen detection, and drug discovery.

查看原文本刊更多论文

利用聚糖阵列和生物传感器来诊断和了解疾病

与正常状态相比，在疾病状态下经常观察到异常的糖基化模式，它参与了诸如癌细胞发展和转移等疾病相关过程。分析聚糖相关的相互作用可以为疾病检测和治疗开发提供有希望的途径。在过去的二十年里，聚糖阵列和生物传感器已经成为表征聚糖相关相互作用、筛选功能性聚糖和检测聚糖相关疾病的强大分析工具。本文综述了用于构建聚糖阵列和生物传感器的聚糖源制备和固定化方法。我们综述了从天然来源获得聚糖的方法以及化学和酶合成的方法。我们特别介绍了一种模拟细胞表面糖结合的DNA、蛋白质和脂质的固定化方法。最后，我们讨论了聚糖阵列和生物传感器在生物医学上的应用，包括癌症特异性聚糖生物标志物的鉴定、癌症中聚糖诱导抗体反应的评估、癌症检测、病原体及其毒素的聚糖结合特异性分析、病原体检测和药物发现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.