Dual-mode colorimetric and fluorometric detection of D-penicillamine via inhibition of peroxidase-mimetic activity of bimetallic N-doped carbon dots

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-06-25 DOI:10.1016/j.saa.2025.126605

Mohamed N. Goda , Laila S. Alqarni , Hossieny Ibrahim , Al-Montaser Bellah H. Ali , Mohamed M. El-Wekil

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Abstract

D-penicillamine (D-PEN) is used to treat Wilson's disease, rheumatoid arthritis, and cystinuria but requires careful monitoring due to its narrow therapeutic window and risks of nephrotoxicity, hematological disorders, and autoimmune reactions. To enable reliable detection, bimetallic FeCu co-doped carbon dots (FeCu@CDs) were developed as multifunctional nanozymes for dual-mode sensing of D-PEN. These nanozymes integrate peroxidase-like catalytic activity, intrinsic fluorescence, and selective thiol-binding affinity, allowing simultaneous colorimetric and ratiometric fluorescence detection. Fe and Cu doping enhanced the peroxidase-like activity and fluorescence emission of the CDs by promoting H₂O₂ activation into HO• and ¹O₂ and improving charge mobility. D-PEN inhibited this catalytic activity by binding metal centers and altered the inner filter effect (IFE) between FeCu@CDs and 2, 3-diaminophenazine (DAP), enabling selective signal modulation. The assay achieved detection limits of 0.13 μM (colorimetric) and 0.03 μM (fluorescence), with recoveries ranging from 95.6 % to 105.1 % in real samples, highlighting its importance in clinical monitoring and bioanalytical applications.

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双模比色法和荧光法通过抑制双金属n掺杂碳点的过氧化物酶模拟活性检测d -青霉胺

d -青霉胺（D-PEN）用于治疗威尔逊氏病、类风湿性关节炎和胱氨酸尿症，但由于其治疗窗口狭窄以及肾毒性、血液系统疾病和自身免疫反应的风险，需要仔细监测。为了实现可靠的检测，开发了双金属FeCu共掺杂碳点（FeCu@CDs）作为多功能纳米酶，用于D-PEN的双模式传感。这些纳米酶整合了过氧化物酶样催化活性，固有荧光和选择性硫醇结合亲和力，允许同时进行比色和比例荧光检测。Fe和Cu掺杂通过促进H₂O₂活化成HO•和O₂，提高电荷迁移率，增强了CDs的类过氧化物酶活性和荧光发射。D-PEN通过结合金属中心抑制了这种催化活性，并改变了FeCu@CDs和2,3 -二氨基吩嗪（DAP）之间的内部过滤效应（IFE），实现了选择性信号调制。该方法的检出限分别为0.13 μM（比色）和0.03 μM（荧光），回收率为95.6% ~ 105.1%，在临床监测和生物分析应用中具有重要意义。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.