The RcBSK7 gene positively regulates drought tolerance in castor (Ricinus communis L.)

IF 5.6 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-06-28 DOI:10.1016/j.indcrop.2025.121408

Yong Zhao , Rui Luo , Huibo Zhao , Guorui Li , Jianjun Di , Li Wen , Pei Lei , Ximei Ji , Fanjuan Meng , Guibin Xue , Fenglan Huang

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引用次数: 0

Abstract

Drought stress limits the growth, development, and yield of castor. Brassinosteroid (BR)-signalling kinases (BSKs) play important roles in plant responses to abiotic stress. However, it is not yet clear which BSK gene is involved in the response of castor to drought stress. This study conducted an iTRAQ analysis of castor plant roots under 15 % PEG-6000-induced stress conditions and showed that RcBSK7 is a drought stress-responsive gene. Therefore, the function of the RcBSK7 gene in drought resistance was further investigated. Transgenic RcBSK7-overexpressing Arabidopsis and castor plants exhibited increased drought tolerance and antioxidant activity. Additionally, RcBSK7 overexpression under mannitol and abscisic acid (ABA) stress conditions promoted an increase in the number of Arabidopsis roots. Yeast two-hybrid assays and surface plasmon resonance (SPR) analysis revealed that RcBSK7 interacts with RcSAP3 and that RcSAP3, upon interaction with RcBSK7, positively influences drought resistance. Under drought stress, RcBSK7-overexpressing castor plants showed significantly increased expression of the key ABA biosynthetic enzymes NCED and AAO, leading to enhanced ABA accumulation. This study suggests that RcBSK7 positively regulates drought resistance by modulating the ABA signalling pathway, thereby increasing drought tolerance in plants. These findings reveal potential candidate genes for the molecular breeding of castor in response to drought stress.

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RcBSK7基因正调控蓖麻（Ricinus communis L.）耐旱性

干旱胁迫限制了蓖麻的生长发育和产量。油菜素内酯（BR）信号激酶（BSKs）在植物对非生物胁迫的响应中起着重要作用。然而，目前尚不清楚BSK基因与蓖麻对干旱胁迫的反应有关。本研究对蓖麻根系在15% % peg -6000诱导的胁迫条件下进行了iTRAQ分析，结果表明RcBSK7是一个干旱胁迫响应基因。因此，我们将进一步研究RcBSK7基因在抗旱性中的作用。转基因rcbsk7过表达的拟南芥和蓖麻表现出更高的耐旱性和抗氧化活性。此外，甘露醇和ABA胁迫下RcBSK7的过表达促进了拟南芥根系数量的增加。酵母双杂交实验和表面等离子体共振（SPR）分析表明，RcBSK7与RcSAP3相互作用，RcSAP3与RcBSK7相互作用后对抗旱性有正向影响。干旱胁迫下，rcbsk7过表达蓖麻植株ABA关键生物合成酶NCED和AAO的表达显著增加，ABA积累增强。本研究提示RcBSK7通过调控ABA信号通路正向调控抗旱性，从而提高植物的抗旱性。这些发现揭示了蓖麻干旱胁迫分子育种的潜在候选基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.