Roles of multiple TEF30-associated intermediate complexes in the repair and reassembly of photosystem II in Chlamydomonas reinhardtii

IF 15.8 1区 生物学 Q1 PLANT SCIENCES
Yidi Wang, Chenxi Wang, Anjie Li, Zhenfeng Liu
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引用次数: 0

Abstract

During oxygenic photosynthesis, photosystem II (PSII) uses light energy for oxidizing water and reducing plastoquinone. It is susceptible to photodamage, and the damaged PSII is repaired through a sophisticated biological process assisted by numerous auxiliary proteins. Here we report the cryogenic electron microscopy structures of four PSII-repair complexes from Chlamydomonas reinhardtii associated with the Thylakoid Enriched Fraction 30 (TEF30, an orthologue of plant MET1) protein—namely, a TEF30–PSII core monomer (TEF30-C), two types of TEF30–PSII core dimers (types I and II, TEF302-C2-I and TEF302-C2-II) and a TEF30-C2S-type PSII–LHCII supercomplex (TEF30-C2S; S, strongly associated light-harvesting complex II trimer). TEF30 mediates the assembly of CP43 with the RC47 module by clamping on the stromal surfaces and prevents the premature association of peripheral antennae with PSII-C. In the transition from TEF302-C2-I to TEF302-C2-II, TEF30-C2S and mature C2S2, one PSII core slides along the dimerization interface against the adjacent one by 22–35 Å, generating a zigzagged surface for accommodating the peripheral antennae. These results suggest that the PSII repair process undergoes multiple TEF30-mediated intermediate states to form intact PSII–LHCII supercomplexes.

多个tef30相关中间复合物在莱茵衣藻光系统II修复和重组中的作用
在含氧光合作用中,光系统II (PSII)利用光能氧化水并还原质体醌。PSII易受光损伤,受损的PSII通过许多辅助蛋白辅助的复杂生物过程进行修复。本文报道了莱因衣藻与类囊体富集部分30 (TEF30,植物MET1的同源物)蛋白相关的四种psii修复复合物的低温电镜结构,即TEF30- psii核心单体(TEF30- c),两种TEF30- psii核心二聚体(I型和II型,TEF302-C2-I和TEF302-C2-II)和TEF30- c2s型PSII-LHCII超复合物(TEF30- c2s;S,强关联的光收集配合物II三聚体)。TEF30通过夹紧基质表面介导CP43与RC47模块的组装,并防止外周天线与PSII-C的过早关联。在从TEF302-C2-I过渡到TEF302-C2-II、TEF30-C2S和成熟的C2S2过程中,一个PSII核心沿着二聚化界面与相邻的PSII核心滑动22-35 Å,形成一个锯齿形表面,用于容纳周边天线。这些结果表明,PSII修复过程经历了多个tef30介导的中间状态,形成完整的PSII - lhcii超复合物。
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来源期刊
Nature Plants
Nature Plants PLANT SCIENCES-
CiteScore
25.30
自引率
2.20%
发文量
196
期刊介绍: Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.
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