Effects of N-Acetyl Cysteine on Human Post-Thaw Sperm Quality and Mitochondrial Uncoupling Protein 2 Relative Quantity.

IF 1.6 4区 生物学
Pegah Dorodian, Abdolhossein Shahverdi, AliReza Alizadeh, Leila Rashki Ghaleno, Shima Abbasihormozi, Vahid Esmaeili, Vahid Akbarinejad, Mohsen Sharafi
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Abstract

Reactive oxygen species (ROS) during cryopreservation causes mechanical, biochemical, and structural damage to the sperm, which leads to reduced sperm motility and fertility. N-acetyl cysteine is a cysteine-derived amino acid antioxidant that functions as a scavenger of ROS and regulates mitochondrial activity. Mitochondrial uncoupling protein 2 (UCP2) plays a leading role in this process and is one of the major regulators of human spermatozoa motility and metabolism. The purpose of the study was to examine the changes in UCP2 in frozen-thawed human sperm when exposed to N-acetyl cysteine, an effective antioxidant commonly used in human semen freezing. Semen samples were collected from 20 normozoospermia men and were divided into four experimental groups: fresh, frozen control, frozen N-Acetylcysteine (NAC, 100 μM), and frozen negative control with Genipin (25 μM). Subsequently, post-thaw sperm quality parameters, as well as UCP2 relative quantity, ROS, mitochondrial membrane potential (MMP), and malondialdehyde, were assessed. Semen treated with NAC exhibited significantly higher total and progressive motility, as well as viability, when compared to the control and genipin groups (p < 0.05). Moreover, UCP2 relative quantity was significantly lower in all frozen groups compared to the fresh group (p < 0.0001). The UCP2 relative quantity was not significantly different between NAC and control groups (p ≥ 0.05). Also, there were no significant differences in MMP, ROS, and malondialdehyde levels among the frozen groups (p ≥ 0.05). It can be concluded that UCP2 undergoes a modification during cryopreservation, and it could be an explanation of the reduction in post-thaw motility of sperm. Additionally, NAC supplementation in freezing media enhances post-thaw sperm motility and viability.

n -乙酰半胱氨酸对人解冻后精子质量和线粒体解偶联蛋白2相对数量的影响
低温保存过程中的活性氧(ROS)对精子造成机械、生化和结构损伤,导致精子活力和生育能力下降。n -乙酰半胱氨酸是一种半胱氨酸衍生的氨基酸抗氧化剂,具有清除活性氧和调节线粒体活性的功能。线粒体解偶联蛋白2 (UCP2)在这一过程中起主导作用,是人类精子运动和代谢的主要调节因子之一。这项研究的目的是研究当暴露于n-乙酰半胱氨酸(一种通常用于人类精液冷冻的有效抗氧化剂)时,冷冻解冻的人类精子中UCP2的变化。采集20例正常精子症男性的精液样本,分为新鲜、冷冻对照组、冷冻n -乙酰半胱氨酸(NAC, 100 μM)和用Genipin (25 μM)冷冻阴性对照组4个实验组。随后,评估解冻后精子质量参数,以及UCP2相对数量、ROS、线粒体膜电位(MMP)和丙二醛。与对照组和genipin组相比,NAC处理的精液总活力、进行活力和活力显著提高(p < 0.05)。此外,所有冷冻组的UCP2相对数量均显著低于新鲜组(p < 0.0001)。NAC组与对照组UCP2相对数量差异无统计学意义(p≥0.05)。MMP、ROS、丙二醛水平在冷冻组间差异无统计学意义(p≥0.05)。由此可以得出结论,UCP2在冷冻保存过程中发生了修饰,这可能是精子解冻后活力降低的原因之一。此外,在冷冻培养基中补充NAC可以提高解冻后精子的活力和生存能力。
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来源期刊
Biopreservation and Biobanking
Biopreservation and Biobanking Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology
自引率
12.50%
发文量
114
期刊介绍: Biopreservation and Biobanking is the first journal to provide a unifying forum for the peer-reviewed communication of recent advances in the emerging and evolving field of biospecimen procurement, processing, preservation and banking, distribution, and use. The Journal publishes a range of original articles focusing on current challenges and problems in biopreservation, and advances in methods to address these issues related to the processing of macromolecules, cells, and tissues for research. In a new section dedicated to Emerging Markets and Technologies, the Journal highlights the emergence of new markets and technologies that are either adopting or disrupting the biobank framework as they imprint on society. The solutions presented here are anticipated to help drive innovation within the biobank community. Biopreservation and Biobanking also explores the ethical, legal, and societal considerations surrounding biobanking and biorepository operation. Ideas and practical solutions relevant to improved quality, efficiency, and sustainability of repositories, and relating to their management, operation and oversight are discussed as well.
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