Early life programming of the neonatal bovine jejunum in response to maternal vitamin and mineral supplementation.

IF 1.8 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Audrey J Craner, Carl R Dahlen, Jennifer L Hurlbert, Ana Clara B Menezes, Priyanka Banerjee, Friederike Baumgaertner, Kerri A Bochantin-Winders, Samat Amat, Kevin K Sedivec, Kendall C Swanson, Wellison J S Diniz
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引用次数: 0

Abstract

We investigated the effects of maternal vitamin and mineral supplementation throughout gestation on gene expression in the jejunal mucosa of neonatal calves. Crossbred Angus heifers (n = 14) were estrus synchronized, bred to female-sexed semen, and randomly assigned to a basal diet (Control, CON; n = 7) or the basal diet plus vitamin and mineral supplement (Treatment, VTM; n = 7). After parturition, calves were removed from their dams before suckling, fed colostrum replacer, and euthanized 30 h after the first feeding. A subsample of the mucosa of the mid-jejunum was collected, and total RNA was isolated. Gene expression was measured using RNA-Seq, and differentially expressed genes (DEGs) were identified using DESeq2. We identified 528 DEGs from the jejunal mucosa between the VTM and CON calves (P ≤ 0.05 and |log2FC| ≥ 0.5). The DEGs were associated with nutrient transport, lipid metabolism, and immune-related biological processes and pathways. Interestingly, genes underlying the complement and coagulation cascades were mostly downregulated in calves born to VTM dams. On the other hand, the cytokine-cytokine receptor interaction KEGG pathway showed most genes upregulated (LIFR, KDR, TNFRSF4, TNFSF18, FLT1, and TNFRSF12A). Our results show that vitamin and mineral supplementation throughout gestation affects genes underlying tissue structure, nutrient transport and metabolism, and immune system pathways in neonates. The implications of such changes and the long-term outcomes on herd health and performance warrant further research.

新生儿牛空肠对母体维生素和矿物质补充的早期生命规划。
我们研究了妊娠期间母体补充维生素和矿物质对新生犊牛空肠黏膜基因表达的影响。杂交安格斯小母牛(n = 14)同步发情,与雌性精液杂交,随机分配为基础日粮(对照组,对照组;n = 7)或基础饮食加维生素和矿物质补充剂(治疗,VTM;N = 7)。分娩后,犊牛在哺乳前离开犊牛,饲喂初乳替代品,第一次饲喂30 h后安乐死。收集中段空肠黏膜亚样本,分离总RNA。采用RNA-Seq检测基因表达,采用DESeq2检测差异表达基因(DEGs)。我们从VTM和CON犊牛之间的空肠黏膜中鉴定出528个deg (P≤0.05,|log2FC|≥0.5)。deg与营养转运、脂质代谢和免疫相关的生物过程和途径有关。有趣的是,补体和凝血级联反应背后的基因在VTM坝出生的小牛中大多下调。另一方面,细胞因子-细胞因子受体相互作用KEGG通路显示大部分基因上调(LIFR、KDR、TNFRSF4、TNFSF18、FLT1和TNFRSF12A)。我们的研究结果表明,在整个妊娠期补充维生素和矿物质会影响新生儿组织结构、营养转运和代谢以及免疫系统途径的基因。这些变化的影响以及对畜群健康和性能的长期影响值得进一步研究。
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来源期刊
Journal of Developmental Origins of Health and Disease
Journal of Developmental Origins of Health and Disease PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-
CiteScore
3.80
自引率
0.00%
发文量
145
审稿时长
6-12 weeks
期刊介绍: JDOHaD publishes leading research in the field of Developmental Origins of Health and Disease (DOHaD). The Journal focuses on the environment during early pre-natal and post-natal animal and human development, interactions between environmental and genetic factors, including environmental toxicants, and their influence on health and disease risk throughout the lifespan. JDOHaD publishes work on developmental programming, fetal and neonatal biology and physiology, early life nutrition, especially during the first 1,000 days of life, human ecology and evolution and Gene-Environment Interactions. JDOHaD also accepts manuscripts that address the social determinants or education of health and disease risk as they relate to the early life period, as well as the economic and health care costs of a poor start to life. Accordingly, JDOHaD is multi-disciplinary, with contributions from basic scientists working in the fields of physiology, biochemistry and nutrition, endocrinology and metabolism, developmental biology, molecular biology/ epigenetics, human biology/ anthropology, and evolutionary developmental biology. Moreover clinicians, nutritionists, epidemiologists, social scientists, economists, public health specialists and policy makers are very welcome to submit manuscripts. The journal includes original research articles, short communications and reviews, and has regular themed issues, with guest editors; it is also a platform for conference/workshop reports, and for opinion, comment and interaction.
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