Metabolic and Biochemical Responses of Juvenile Babylonia areolata to Hypoxia Stress.

IF 3.6 3区 生物学 Q1 BIOLOGY
Baojun Tang, Xiaoyao Ren, Zhiguo Dong, Hanfeng Zheng, Yujia Liu, Tao Wei
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引用次数: 0

Abstract

As an important aquaculture species, the marine snail Babylonia areolata is frequently subjected to fluctuation in dissolved oxygen concentration during farming and transportation processes. In the present study, we investigated the metabolic rates, transcription, and enzyme level responses of juvenile B. areolata exposed to long-term stress (144 h). The results showed that the mortality rate of juvenile B. areolata was higher in the 0.5 mg O2/L group compared to the 2 mg O2/L group. During the hypoxic stress period, both oxygen consumption and ammonia excretion rates were observed to be lower in juvenile B. areolata than those in the control group. As hypoxic stress duration prolonged, juvenile B. areolata demonstrated significantly elevated activities of pyruvate kinase (PK) and alkaline phosphatase (AKP), alongside reduced activities of lactic dehydrogenase (LDH), acid phosphatase (ACP), and superoxide dismutase (SOD). Significant changes in the expression levels of PK and LDH genes were observed during the hypoxic stress. The expression levels of ACP and SOD genes were significantly downregulated, while juvenile B. areolata exhibited elevated AKP gene expression levels under 0.5 mg O2/L. Our findings suggest that under long-term exposure to hypoxia, B. areolata failed to maintain energy homeostasis and suffered biochemical disruptions, leading to a reduction in survival. The mortality rate of B. areolata can be substantially decreased by ensuring that transportation time does not exceed 48 h.

雏鸟对缺氧胁迫的代谢和生化反应。
海螺是一种重要的水产养殖物种,在养殖和运输过程中,溶解氧浓度经常受到波动的影响。在本研究中,我们研究了暴露于长期应激(144 h)下的乳乳圆叶藻幼鱼的代谢率、转录和酶水平的反应。结果表明:0.5 mg O2/L组乳霜白僵鱼幼鱼死亡率高于2 mg O2/L组;在低氧胁迫期间,乳晕白鲟幼鱼的耗氧量和氨排泄率均低于对照组。随着缺氧胁迫持续时间的延长,乳晕白藻幼鱼的丙酮酸激酶(PK)和碱性磷酸酶(AKP)活性显著升高,乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)和超氧化物歧化酶(SOD)活性显著降低。在缺氧胁迫过程中,PK和LDH基因的表达水平发生了显著变化。在0.5 mg O2/L环境下,ACP和SOD基因表达水平显著下调,而幼鱼AKP基因表达水平升高。我们的研究结果表明,在长期缺氧的环境下,乳晕白藻无法维持能量稳态,并遭受生化破坏,导致存活率降低。确保运输时间不超过48 h,可大大降低乳霜白僵菌的死亡率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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