{"title":"Metabolic and Biochemical Responses of Juvenile <i>Babylonia areolata</i> to Hypoxia Stress.","authors":"Baojun Tang, Xiaoyao Ren, Zhiguo Dong, Hanfeng Zheng, Yujia Liu, Tao Wei","doi":"10.3390/biology14060727","DOIUrl":null,"url":null,"abstract":"<p><p>As an important aquaculture species, the marine snail <i>Babylonia areolata</i> is frequently subjected to fluctuation in dissolved oxygen concentration during farming and transportation processes. In the present study, we investigated the metabolic rates, transcription, and enzyme level responses of juvenile <i>B. areolata</i> exposed to long-term stress (144 h). The results showed that the mortality rate of juvenile <i>B. areolata</i> was higher in the 0.5 mg O<sub>2</sub>/L group compared to the 2 mg O<sub>2</sub>/L group. During the hypoxic stress period, both oxygen consumption and ammonia excretion rates were observed to be lower in juvenile <i>B. areolata</i> than those in the control group. As hypoxic stress duration prolonged, juvenile <i>B. areolata</i> demonstrated significantly elevated activities of pyruvate kinase (PK) and alkaline phosphatase (AKP), alongside reduced activities of lactic dehydrogenase (LDH), acid phosphatase (ACP), and superoxide dismutase (SOD). Significant changes in the expression levels of <i>PK</i> and <i>LDH</i> genes were observed during the hypoxic stress. The expression levels of <i>ACP</i> and <i>SOD</i> genes were significantly downregulated, while juvenile <i>B. areolata</i> exhibited elevated <i>AKP</i> gene expression levels under 0.5 mg O<sub>2</sub>/L. Our findings suggest that under long-term exposure to hypoxia, <i>B. areolata</i> failed to maintain energy homeostasis and suffered biochemical disruptions, leading to a reduction in survival. The mortality rate of <i>B. areolata</i> can be substantially decreased by ensuring that transportation time does not exceed 48 h.</p>","PeriodicalId":48624,"journal":{"name":"Biology-Basel","volume":"14 6","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12190147/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biology-Basel","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/biology14060727","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
As an important aquaculture species, the marine snail Babylonia areolata is frequently subjected to fluctuation in dissolved oxygen concentration during farming and transportation processes. In the present study, we investigated the metabolic rates, transcription, and enzyme level responses of juvenile B. areolata exposed to long-term stress (144 h). The results showed that the mortality rate of juvenile B. areolata was higher in the 0.5 mg O2/L group compared to the 2 mg O2/L group. During the hypoxic stress period, both oxygen consumption and ammonia excretion rates were observed to be lower in juvenile B. areolata than those in the control group. As hypoxic stress duration prolonged, juvenile B. areolata demonstrated significantly elevated activities of pyruvate kinase (PK) and alkaline phosphatase (AKP), alongside reduced activities of lactic dehydrogenase (LDH), acid phosphatase (ACP), and superoxide dismutase (SOD). Significant changes in the expression levels of PK and LDH genes were observed during the hypoxic stress. The expression levels of ACP and SOD genes were significantly downregulated, while juvenile B. areolata exhibited elevated AKP gene expression levels under 0.5 mg O2/L. Our findings suggest that under long-term exposure to hypoxia, B. areolata failed to maintain energy homeostasis and suffered biochemical disruptions, leading to a reduction in survival. The mortality rate of B. areolata can be substantially decreased by ensuring that transportation time does not exceed 48 h.
期刊介绍:
Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.