Spectral analysis of protonated and deprotonated form of ribonucleotides and their components at room temperature.

Abstract

Spectral studies of nucleotides are typically conducted for their deprotonated forms; consequently, there is a paucity of knowledge regarding the fluorescent properties of protonated nucleotide forms. Therefore, this work aimed to analyze and compare their spectral properties under conditions close to biological systems. We studied the absorption, excitation and emission of protonated and deprotonated forms of monoribonucleotides and their components dissolved in water at room temperature. From the data obtained, we calculated values for the energies of the first excited singlet electronic levels of the de- and protonated forms of nucleotides and their constituents. The most significant difference between the energies of the first excited states of the protonated and deprotonated forms of nucleotides was observed for uridine monophosphate. We observed a change in the ratio of bands in the absorption, excitation and fluorescence spectra of protonated and deprotonated forms of nucleotides.