Investigation and genetic confirmation of the Cryptosporidium species in fish handlers in Baghdad city.

IF 1.5 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Advanced Veterinary and Animal Research Pub Date : 2025-03-22 eCollection Date: 2025-03-01 DOI:10.5455/javar.2025.l866

Noor Majed Obead, Amer Rasool Alhaboubi

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Abstract

Objective: The present study aims to investigate molecular confirmation for Cryptosporidium species in fish handlers in Baghdad City, central Iraq.

Materials and methods: Sixty stool samples were collected between early November 2023 and late April 2024. All samples were examined phenotypically using a modified Ziehl-Neelsen stain and genotypically (nested polymerase chain reaction technique) based on a partial sequence of 18S rRNA genes with sequencing and phylogenetic tree analysis.

Results: The total molecular results identified Cryptosporidium parvum with an infection rate of 45% (27/60). A higher infection rate of 51.9% (14/27) was found in the age group between 15 and 35 years, and male handlers recorded a lower infection rate (45%) than females (41.6%). April had a higher elevation in the infection rate of 60% (6/10) than other months.

Conclusion: The C. parvum was the only species found in fish handlers, and these local isolates have higher similarity with other isolates of China and Iran.

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巴格达市鱼类加工者隐孢子虫种类的调查和遗传确认。

目的：对伊拉克中部巴格达市鱼工中隐孢子虫的种类进行分子鉴定。材料与方法：于2023年11月初至2024年4月底收集粪便样本60份。所有样品均采用改良的Ziehl-Neelsen染色进行表型检测，并基于18S rRNA基因的部分序列进行基因检测（巢式聚合酶链反应技术），并进行测序和系统发育树分析。结果：总分子结果鉴定出细小隐孢子虫，感染率为45%（27/60）。15 ~ 35岁年龄组感染率较高，为51.9%(14/27)，男性处理者感染率（45%）低于女性（41.6%）。4月感染率升高60%(6/10)，高于其他月份。结论：在鱼处理人员中发现的唯一一种是小弧菌，这些地方分离株与中国和伊朗的其他分离株具有较高的相似性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Advanced Veterinary and Animal Research Veterinary-Veterinary (all)

CiteScore

2.70

自引率

7.10%

发文量

审稿时长

6 weeks

期刊介绍： Journal of Advanced Veterinary and Animal Research (JAVAR) - is an open access, international, peer-reviewed, quarterly, highly-indexed scientific journal publishing original research findings and reviews on all aspects of veterinary and animal sciences. Basic and applied researches on- - Anatomy & histology - Animal health economics - Animal nutrition - Animal reproduction - Animal science - Antimicrobial resistance (AMR) - Biochemistry - Biotechnology - Dairy science - Epidemiology - Food hygiene and technology - Genetics and breeding - Immunology - Microbiology - Parasitology - Pathology - Pharmacology & toxicology - Physiology - Poultry science - Preventive veterinary medicine - Public health - Surgery & obstetrics - Veterinary extension studies - Wildlife & aquatic medicine - Zoo animal medicine.