Molecular functional mechanisms of two alcohol acetyltransferases in Lavandula x intermedia (lavandin).

IF 3.8 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Frontiers in Chemistry Pub Date : 2025-06-11 eCollection Date: 2025-01-01 DOI:10.3389/fchem.2025.1627286

Dafeng Liu, Yanyan Du, Ablikim Abdiriyim, Lvxia Zhang, Daoqi Song, Huashui Deng, Xiongying Wen, Yanyan Zhang, Bingwang Sun

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Abstract

Volatile esters are key flavor components in most plants, including Lavandula x intermedia (lavandin). The final step in ester biosynthesis is catalyzed by Lavandula x intermedia alcohol acyltransferases (LiAATases), which attach alcohols to acyl groups. However, the functional role and mechanism of LiAATases remain poorly understood. Here, we predicted their structural models using AlphaFold2 and identified potential active site residues through the GalaxyWEB program. Catalytic assays were optimized at pH 7.5 and 30 °C. Substrate specificity for alcohols was assessed for both enzymes. Gene expression analysis revealed that LiAATase1 and LiAATase2 were most highly expressed in the petals and pistils, respectively, with peak expression occurring at stage 4 for LiAATase1 and stage 1 for LiAATase2. Our study aims to elucidate the functional properties of alcohol acyltransferases in Lavandula x intermedia, contributing to an understanding of ester biosynthesis and specificity in this species.

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薰衣草中两种醇乙酰转移酶的分子功能机制

挥发性酯是大多数植物的主要风味成分，包括薰衣草。酯生物合成的最后一步是由Lavandula x中间醇酰基转移酶（LiAATases）催化，该酶将醇连接到酰基上。然而，人们对LiAATases的功能作用和机制仍然知之甚少。在这里，我们使用AlphaFold2预测了它们的结构模型，并通过GalaxyWEB程序识别了潜在的活性位点残基。催化实验在pH 7.5和30℃条件下进行优化。评估了两种酶对醇的底物特异性。基因表达分析表明，LiAATase1和LiAATase2分别在花瓣和雌蕊中表达量最高，其中LiAATase1和LiAATase2分别在第4期和第1期达到表达高峰。我们的研究旨在阐明薰衣草中醇酰基转移酶的功能特性，有助于了解该物种的酯生物合成和特异性。

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来源期刊

Frontiers in Chemistry Chemistry-General Chemistry

CiteScore

8.50

自引率

3.60%

发文量

1540

审稿时长

12 weeks

期刊介绍： Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide. Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”. All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.