Unraveling the Multi-Omic Landscape of Extracellular Vesicles in Human Seminal Plasma.

IF 4.8 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomolecules Pub Date : 2025-06-07 DOI:10.3390/biom15060836

Laura Governini, Alesandro Haxhiu, Enxhi Shaba, Lorenza Vantaggiato, Alessia Mori, Marco Bruttini, Francesca Loria, Natasa Zarovni, Paola Piomboni, Claudia Landi, Alice Luddi

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引用次数: 0

Abstract

Extracellular Vesicles (EVs) from seminal plasma have achieved attention due to their potential physiopathological role in male reproductive systems. This study employed a comprehensive proteomic and transcriptomic approach to investigate the composition and molecular signatures of EVs isolated from human seminal plasma. EVs from Normozoospermic (NORMO), OligoAsthenoTeratozoospermic (OAT), and Azoospermic (AZO) subjects were isolated using a modified polymer precipitation-based protocol and characterized for size and morphology. Comprehensive proteomic analysis, using both gel-free and gel-based approaches, revealed distinct protein profiles in each group (p<0.01), highlighting potential molecules and pathways involved in sperm function and fertility. The data are available via ProteomeXchange with identifiers PXD051361 and PXD051390, respectively. Transcriptomic analysis confirmed the trend of a general downregulation of AZO and OAT compared to NORMO shedding light on regulatory mechanisms of sperm development. Bioinformatic tools were applied for functional omics analysis; the integration of proteomic and transcriptomic data provided a comprehensive understanding of the cargo content and regulatory networks present in EVs. This study contributes to elucidating the key role of EVs in the paracrine communication regulating spermatogenesis. A full understanding of these pathways not only suggests potential mechanisms regulating male fertility but also offers new insights into the development of diagnostic tools targeting male reproductive disorders.

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揭示人类精浆中细胞外囊泡的多组学景观。

精浆细胞外囊泡（EVs）因其在男性生殖系统中潜在的生理病理作用而受到关注。本研究采用综合的蛋白质组学和转录组学方法研究了从人精浆中分离的ev的组成和分子特征。采用基于改性聚合物沉淀的方法从正常精子（NORMO）、低弱畸形精子（OAT）和无精子（AZO）受试者中分离出ev，并对其大小和形态进行了表征。综合蛋白质组学分析，使用无凝胶和基于凝胶的方法，揭示了每组不同的蛋白质谱(p

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来源期刊

Biomolecules Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

9.40

自引率

3.60%

发文量

1640

审稿时长

18.28 days

期刊介绍： Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.