Evaluation of the potential therapeutic effect of africanized honeybee venom (Apis mellifera Linnaeus) on levodopa-induced dyskinesias in mice.

IF 1.1 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Anais da Academia Brasileira de Ciencias Pub Date : 2025-06-20 eCollection Date: 2025-01-01 DOI:10.1590/0001-3765202520240826
Camila G Dantas, Adriano A S Araújo, Ricardo Luiz C DE Albuquerque Junior, Edna A F Cândido, Margarete Z Gomes, Luiz P DA Costa
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引用次数: 0

Abstract

Levodopa-induced dyskinesia (LID) is a complication in patients with Parkinson's disease and undergoing long-term levodopa replacement therapy. This study evaluated the the potential therapeutic effect of Africanized honey bee venom (Apis mellifera L.) on LID in mice. Prior to bee venom (BV) or amantadine treatment, mice received medial forebrain bundle microinjections of 6-hydroxydopamine or ascorbate saline. The animals received dose of levodopa (6mg.Kg-1 for 5 days and 12mg.Kg-1 for 10 days) combined with a dose of 12 mg.Kg-1 of benserazide, for 15 days. Amantadine (40mg.Kg-1) or BV were administered at different dosages (0.1; 0.5 or 1mg.Kg-1) once every 2 days, starting from the last day of levodopa administration, for 2 weeks. The evaluation of abnormal involuntary movements, cylinder test, open field, rotational behavior and wire suspension were done on the day following the last administration of treatment. Was carried the histopathological analysis. The chemical composition of BV was also assessed, identifying the highest concentrations of apamin, phospholipase A2 and melittin and the antioxidant activity. Treatment with BV at a dose of 0.1mg.kg-1 reduced apomorphine-induced rotations, increased the number of contralateral contacts to the lesion, and increased grip strength, restoring motor control impaired by LIDs, indicating potential therapeutic efficacy.

非洲化蜂毒(Apis mellifera Linnaeus)对左旋多巴诱导的小鼠运动障碍潜在治疗效果的评价。
左旋多巴诱导的运动障碍(LID)是长期接受左旋多巴替代治疗的帕金森病患者的并发症。本研究评价了非洲化蜂毒(Apis mellifera L.)治疗小鼠LID的潜在疗效。在蜂毒(BV)或金刚烷胺治疗之前,小鼠接受内侧前脑束显微注射6-羟多巴胺或抗坏血酸盐盐水。小鼠给予左旋多巴6mg。Kg-1, 5天,12毫克。Kg-1,持续10天),外加12毫克的剂量。Kg-1的苯塞拉肼,持续15天。金刚烷胺(40mg.Kg-1)或BV按不同剂量(0.1;0.5或1mg.Kg-1)每2天1次,自左旋多巴给药最后一天起,连续2周。在最后一次给药后的第二天进行异常不自主运动、圆柱体试验、空地、旋转行为和钢丝悬吊的评估。进行组织病理学分析。测定了BV的化学成分,确定了最高浓度的维生素、磷脂酶A2和蜂毒素以及抗氧化活性。用BV治疗,剂量为0.1mg。kg-1减少阿帕吗啡诱导的旋转,增加对侧与病变的接触次数,增加握力,恢复眼睑受损的运动控制,表明潜在的治疗效果。
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来源期刊
Anais da Academia Brasileira de Ciencias
Anais da Academia Brasileira de Ciencias 综合性期刊-综合性期刊
CiteScore
2.20
自引率
0.00%
发文量
347
审稿时长
1 months
期刊介绍: The Brazilian Academy of Sciences (BAS) publishes its journal, Annals of the Brazilian Academy of Sciences (AABC, in its Brazilianportuguese acronym ), every 3 months, being the oldest journal in Brazil with conkinuous distribukion, daking back to 1929. This scienkihic journal aims to publish the advances in scienkihic research from both Brazilian and foreigner scienkists, who work in the main research centers in the whole world, always looking for excellence. Essenkially a mulkidisciplinary journal, the AABC cover, with both reviews and original researches, the diverse areas represented in the Academy, such as Biology, Physics, Biomedical Sciences, Chemistry, Agrarian Sciences, Engineering, Mathemakics, Social, Health and Earth Sciences.
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