X-ray fluorescence standing wave study of the interaction of the antimicrobial peptide indolicidin with a supported model membrane.

IF 2.9 3区 化学 Q3 CHEMISTRY, PHYSICAL
Soft Matter Pub Date : 2025-06-26 DOI:10.1039/d5sm00257e
Gobind Basnet, Jonathan Maloney, Jyotsana Lal, Elizabeth Gaillard, Denis T Keane, Evguenia A Karapetrova, Raymond Conley, Laurence Lurio
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引用次数: 0

Abstract

Indolicidin, a cationic antimicrobial peptide, interacts with lipid bilayers through electrostatic and hydrophobic interactions, disrupting microbial membranes. We investigated the depth-dependent localization of gold-nanoparticle-labeled indolicidin in a supported model membrane using X-ray fluorescence standing wave (SWXF) analysis. Liposomes composed of DMPC and DMPG were incubated with indolicidin labeled at its C-terminus with a 1.8 nm gold nanoparticle, then deposited onto a Si/Mo multilayer substrate via vesicle bursting. SWXF measurements revealed that at low peptide incubation concentrations (2-5 μM), gold-nanoparticle-labeled indolicidin remains primarily associated with the bilayer's outer leaflet. At higher concentrations (10 μM), the peptide penetrates deeper into the bilayer, with the labeled C-terminal region either localizing near the membrane's hydrophobic core or inducing membrane breakup. These findings suggest a concentration-dependent insertion mechanism.

抗菌肽吲哚啶与支撑模型膜相互作用的x射线荧光驻波研究。
吲哚啶是一种阳离子抗菌肽,通过静电和疏水相互作用与脂质双分子层相互作用,破坏微生物膜。我们利用x射线荧光驻波(SWXF)分析研究了金纳米颗粒标记的吲哚西丁在支撑模型膜中的深度依赖定位。由DMPC和DMPG组成的脂质体与在c端标记1.8 nm金纳米颗粒的吲哚啶一起孵育,然后通过囊泡破裂沉积在Si/Mo多层衬底上。SWXF测量显示,在低肽培养浓度(2-5 μM)下,金纳米颗粒标记的吲哚啶主要与双分子层的外小叶相关。在较高的浓度(10 μM)下,肽渗透到双层中更深,标记的c端区域要么定位在膜的疏水核心附近,要么诱导膜破裂。这些发现提示了一种浓度依赖的插入机制。
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来源期刊
Soft Matter
Soft Matter 工程技术-材料科学:综合
CiteScore
6.00
自引率
5.90%
发文量
891
审稿时长
1.9 months
期刊介绍: Soft Matter is an international journal published by the Royal Society of Chemistry using Engineering-Materials Science: A Synthesis as its research focus. It publishes original research articles, review articles, and synthesis articles related to this field, reporting the latest discoveries in the relevant theoretical, practical, and applied disciplines in a timely manner, and aims to promote the rapid exchange of scientific information in this subject area. The journal is an open access journal. The journal is an open access journal and has not been placed on the alert list in the last three years.
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