Lei Lei, Jia Liu, Yueting Shi, Yiping Wu, Wei Wu, Yuhan Hu, Haoxuan Wang, Jiaqing Wang, Xingyi Li
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引用次数: 0
Abstract
The rapid corneal re-epithelization after injury is crucial for maintaining corneal homeostasis and barrier function. This study reports a bioadhesive functional peptide-derived supramolecular hydrogel (FBA-FFGGRGD) for promoting corneal re-epithelization. The aldehyde-modified bioactive peptide (RGD) endows the hydrogel with bioadhesive properties, enabling covalent anchoring to corneal basement membrane proteins via Schiff base reaction. Compared with its analogue (TA-FFGGRGD), FBA-FFGGRGD significantly enhances adhesion, migration, and proliferation of human corneal epithelial cells (HCECs), doubling cell numbers within 48 h and upregulating zonula occludens-1 (ZO-1) expression. In vivo studies show that the hydrogel exhibits excellent ocular biocompatibility, prolongs precorneal retention (∼40 min), and promotes stratified epithelium formation in a rabbit model of corneal injury. By remodeling the epithelial barrier, this bioadhesive hydrogel provides a promising strategy for corneal homeostasis preservation and ocular disorder prevention.
期刊介绍:
Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine.
Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.